Laboratory of Biotechnology

Code:

BIOL4010

Acronym:

BIOL4010

Keywords
Classification	Keyword
OFICIAL	Biology

Instance: 2023/2024 - 2S

Active?	Yes
Responsible unit:	Department of Biology
Course/CS Responsible:	Master in Functional Biology and Biotechnology of Plants

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
M:BFBP	5	Plano de Estudos M:BFBP_2015_2016	1	-	6	42	162

Teaching language

Suitable for English-speaking students

Objectives

Provide students with basic knowledge of in vitro plant cell cultures and practical applications,  including micropropagation and transformation of plants. Provide students with basic knowledge of manipulation in aseptic conditions.

Learning outcomes and competences

After completion of the course, the student should be able to:

- recognize the various in vitro plant culture techniques, and good practices of in vitro manipulation
- develop methodologies for the establishment of plant cell cultures
- perform the techniques leading to the in vitro plant propagation
- transform plants permanently or transient
- identify innovative applications in plant biotechnology

Working method

Presencial

Pre-requirements (prior knowledge) and co-requirements (common knowledge)

Not applicable

Program

Fundamental notions about of plant cells cultures.
Media and culture conditions.

Use of plant cell cultures for micropropagation using different methodologies.

Permanent Agrobacterium-mediated transformation.

Transient expression of fluorescent fusions protein  in Nicotiana tabacum leaf.

Mandatory literature

Canhoto, J.M.; Biotecnologia Vegetal – da Clonagem de Plantas à Transformação Genética. , Imprensa da Universidade de Coimbra, 2010
Chawla H. S.; Introduction to plant biotechnology. ISBN: 978-1-57808-636-8
Neumann, K-H., Kumar, A. & Imani, J.; Plant Cell and Tissue Culture – a Tool in Biotechnology, Springer-Verlag, Berlin Heidelberg, 2009
Davies Jr FT, Geneve RL, Wilson SB; Hartmann & Kester's Plant Propagation: Principles and Practices, Pearson, 2018. ISBN: 978-0-13448-367-2
Imogen A Sparkes, John Runions, Anne Kearns & Chris Hawes; Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants, NATURE PROTOCOLS | VOL.1 NO.4, 2019-2025, 2006

Complementary Bibliography

Caula A. Beyl; Plant propagation concepts and laboratory exercises. ISBN: 978-1-4665-0387-8

Comments from the literature

The recommended bibliography is available at the FCUP Central library (ed. FC1). Complementary elements of study will also be made available by the teachers of the curricular unit.

Teaching methods and learning activities

Laboratory classes "hands on", providing the acquisition of specific skills, in particular those resulting from the execution of laboratory activities concerning in vitro cultures and plant transformation.

The teaching methodologies include laboratory classes to illustrate major problems and solutions encountered in topics of the curricular unit, allowing the students to perform experiments and to acquire laboratory skills in the in vitro culture and plant transformation fields. In these classes, conducted in an interactive way, is provided to students information and all the conditions to develop laboratory activities involving teamwork, contributing to consolidate their knowledge and develop scientific spirit through analysis and discussion of the results obtained in these activities.

The teaching methodologies will be adjusted in order to allow the students to accomplish the objectives of the curricular unit.

Evaluation Type

Distributed evaluation with final exam

Assessment Components

designation	Weight (%)
Exame	75,00
Participação presencial	25,00
Total:	100,00

Amount of time allocated to each course unit

designation	Time (hours)
Estudo autónomo	120,00
Frequência das aulas	38,00
Apresentação/discussão de um trabalho científico	4,00
Total:	162,00

Eligibility for exams

Compulsory attendance of 75% of the classes provided

Calculation formula of final grade

Not applicable

Classification improvement

It is mandatory to attend the practical classes - minimum 75%.

Observations

Professor coordinator: Fernanda Fidalgo