Fish and chips - ifast microfluidic device for E. Coli O157:H7 capture and detection via on-chip fish assay

• Tipo: Artigo em Livro de Atas de Conferência Internacional
• Data: 2019

Autores

Rodriguez Mateos, P (Autor) Outra; Rodrigues, CF (Autor) Outra; Azevedo, NF (Autor) FEUP; Almeida, C (Autor) Outra; Dyer, CE (Autor) Outra; Iles, A (Autor) Outra; Pamme, N (Autor) Outra

Ata de Conferência Internacional

Título: 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Páginas: 839-840

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

27 October 2019 through 31 October 2019

Indexação

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00T-14Z

Abstract (EN): We explore a simple approach to carry out fluorescence in situ hybridization (FISH) on-chip: combining on-chip magnetic capture of Escherichia coli based on immiscible-filtration-assisted-by-surface-tension (IFAST), followed by detection of the isolated E. coli with FISH in the same device. Preliminary experiments show successful pathogen capture followed by a partial on-chip FISH assay using 107-109 cells mL-1 fixed and permeabilized in three conditions: (1) 4% vol/vol paraformaldehyde and 50% EtOH, (2) 50% EtOH, and (3) 100% EtOH. © 2019 CBMS-0001.

Idioma: Inglês

Tipo (Avaliação Docente): Científica