Fish and chips - ifast microfluidic device for E. Coli O157:H7 capture and detection via on-chip fish assay

• Type: Article in International Conference Proceedings Book
• Date: 2019

Authors

Rodriguez Mateos, P (Author) Other; Rodrigues, CF (Author) Other; Azevedo, NF (Author) FEUP; Almeida, C (Author) Other; Dyer, CE (Author) Other; Iles, A (Author) Other; Pamme, N (Author) Other

Conference proceedings International

Title: 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Pages: 839-840

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

27 October 2019 through 31 October 2019

Indexing

Scopus - 0 Citations

Other information

Authenticus ID: P-00T-14Z

Abstract (EN): We explore a simple approach to carry out fluorescence in situ hybridization (FISH) on-chip: combining on-chip magnetic capture of Escherichia coli based on immiscible-filtration-assisted-by-surface-tension (IFAST), followed by detection of the isolated E. coli with FISH in the same device. Preliminary experiments show successful pathogen capture followed by a partial on-chip FISH assay using 107-109 cells mL-1 fixed and permeabilized in three conditions: (1) 4% vol/vol paraformaldehyde and 50% EtOH, (2) 50% EtOH, and (3) 100% EtOH. © 2019 CBMS-0001.

Language: English

Type (Professor's evaluation): Scientific