Coimmobilization of L-asparaginase and glutamate dehydrogenases onto highly activated supports

• Tipo: Artigo em Revista Científica Internacional
• Data: 2001

Autores

Balcao, VM (Autor) Outra; Mateo, C (Autor) Outra; Fernandez Lafuente, R (Autor) Outra; F. Xavier Malcata (Autor) Outra; Guisan, JM (Autor) Outra

Revista

Título: Enzyme and Microbial Technology

Vol. 28

Páginas: 696-704

ISSN: 0141-0229

Editora: Elsevier

Indexação

ISI Web of Knowledge - 32 Citações

Scopus - 33 Citações

Outras Informações

ID Authenticus: P-000-VHS

DOI: 10.1016/s0141-0229(01)00307-6

Abstract (EN): In the present research work, production of coimmobilized derivatives of L-asparaginase and glutamate dehydrogenase was attempted. Comparison of immobilization of each enzyme independently with coimmobilization of the two enzymes unfolded important advantages of the Latter, namely a decrease in the induction period (time before the maximum reaction rate is virtually achieved! and an increase in the maximum reaction rate. The effectiveness of the independent enzyme derivatives was low; however, it was enhanced by three-fold when the enzymes were coimmobilized onto the same agarose-glutaraldehyde support. Each supporting agarose bead may in fact be viewed as a nano-reactor with in situ reaction and separation (i.e. elimination of the ammonia formed), with the nanoenvironment surrounding each enzyme molecule being essentially devoid of steric hindrance.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9