Microrheological characterisation of Cyanoflan in human blood plasma

• Type: Article in International Scientific Journal
• Date: 2024-02

Authors

Campo Deano, L (Author) FEUP; Rodrigues, T. (Author) FEUP; Mota, R. (Author) Other; Gales, L. (Author) Other; Tamagnini, P (Author) Other

Journal

Title: Carbohydrate Polymers

Vol. 326

ISSN: 0144-8617

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 0 Citations

Other information

Authenticus ID: P-00Z-HEV

DOI: 10.1016/j.carbpol.2023.121575

Abstract (EN): Naturally occurring polysaccharidic biopolymers released by marine cyanobacteria are of great interest for numerous biomedical applications, such as wound healing and drug delivery. Such polymers generally exhibit high molecular weight and an entangled structure that impact the rheology of biological fluids. However, biocompatibility tests focus not so much on rheological properties as on immune response. In the present study, the rheological behaviour of native blood plasma as a function of the concentration of a cyanobacterium biopolymer is investigated via multiple particle tracking microrheology, which measures the Brownian motion of probes embedded in a sample, and cryogenic scanning electron microscope microstructural characterisation. We use Cyanoflan as the biopolymer of choice, and profit from our knowledge of its chemical structure and its exciting potential for biotechnological applications. A sol-gel transition is identified using time-concentration superposition and the power-law behaviour of the incipient network's viscoelastic response is observed in a variety of microrheological data. Our results point to rheology-based principles for blood compatibility tests by facilitating the assignment of quantitative values to specific properties, as opposed to more heuristic approaches.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12