Evaluation of the effect of the degree of acetylation on the inflammatory response to 3D porous chitosan scaffolds

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Judite N. Barbosa (Author) FEUP; Isabel F. Amaral (Author) FEUP; Artur P. Águas (Author) FEUP; Mário A. Barbosa (Author) FEUP

Journal

Title: Journal of Biomedical Materials Research - Part A

Pages: 20-28

ISSN: 1549-3296

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 50 Citations

ISI Web of Science

Scopus - 64 Citations

COMPENDEX

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

CORDIS: Technological sciences > Engineering > Biomaterial engineering

Other information

Authenticus ID: P-003-7W6

DOI: 10.1002/jbm.a.32499

Abstract (EN): The effect of the degree of acetylation (DA) of 3D chitosan (Ch) scaffolds on the inflammatory reaction was investigated. Chitosan porous scaffolds with DAs of 4 and 15% were implanted using a subcutaneous air-pouch model of inflammation. The initial acute inflammatory response was evaluated 24 and 48 h after implantation. To characterize the initial response, the recruitment and adhesion of inflammatory cells to the implant site was studied. The fibrous capsule formation and the infiltration of inflammatory cells within the scaffolds were evaluated for longer implantation times (2 and 4 weeks). Chitosan with DA 15% attracted the highest number of leukocytes to the implant site. High numbers of adherent inflammatory cells were also observed in this material. For longer implantation periods Ch scaffolds with a DA of 15% induced the formation of a thick fibrous capsule and a high infiltration of inflammatory cells within the scaffold. Our results indicate that the biological response to implanted Ch scaffolds was influenced by the DA. Chitosan with a DA of 15% induce a more intense inflammatory response when compared with DA 4% Ch. Because inflammation and healing are interrelated, this result may provide clues for the relative importance of acetyl and amine functional groups in tissue repair and regeneration.

Language: English

Type (Professor's evaluation): Scientific