Novel cerium doped glass-reinforced hydroxyapatite with antibacterial and osteoconductive properties for bone tissue regeneration

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Morais DS (Author) FEUP; Fernandes S (Author) Other; Gomes PS (Author) FMDUP; Fernandes MH (Author) FMDUP; Sampaio P (Author) Other; Ferraz, MP (Author) Other; Santos, JD (Author) FEUP; Maria Ascensão Lopes (Author) FEUP; Sooraj Hussain N (Author) Other

Journal

Title: Biomedical Materials (Bristol)

Vol. 10 No. 5

ISSN: 1748-6041

Publisher: Institute of Physics Publishing

Indexing

ISI Web of Knowledge - 44 Citations

Scopus - 50 Citations

Pubmed / Medline

Scientific classification

FOS: Engineering and technology > Medical engineering

CORDIS: Technological sciences > Engineering > Biomedical enginnering ; Technological sciences > Engineering > Materials engineering ; Technological sciences > Engineering > Biomaterial engineering

Other information

Authenticus ID: P-00G-VZH

DOI: 10.1088/1748-6041/10/5/055008

Abstract (EN): The aim of this work was to develop a bioactive bone substitute with an effective antibacterial ability based on a cerium (Ce) doped glass-reinforced hydroxyapatite (GR-HA) composite. Developed composites were physicochemically characterized, using x-ray diffraction (XRD) analysis, SEM, energy dispersive x-ray spectroscopy (EDS), and flexural bending strength (FBS) tests. X-ray photoelectron spectroscopy (XPS) analysis was performed to analyze the oxidation state of Ce in the prepared doped glass. The antimicrobial activity of the composites was evaluated against Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa; whether the cytocompatibility profile was assayed with human osteoblastic-like cells (Mg-63 cell line). The results revealed that the Ce inclusion in the GR-HA matrix induced the antimicrobial ability of the composite. In addition, Ce-doped materials reported an adequate biological behavior following seeding of osteoblastic populations, by inducing cell adhesion and proliferation. Developed materials were also found to enhance the expression of osteoblastic-related genes. Overall, the developed GR-HA_Ce composite is a prospective candidate to be used within the clinical scenario with a successful performance due to the effective antibacterial properties and capability of enhancing the osteoblastic cell response.

Language: English

Type (Professor's evaluation): Scientific