Surface functionalization of polypropylene (PP) by chitosan immobilization to enhance human fibroblasts viability

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Diana S. Morais (Author) Other; B. Ávila (Author) Other; C. Lopes (Author) Other; Miguel A. Rodrigues (Author) Other; Filipe Vaz (Author) Other; Vera A. Machado (Author) Other; Maria Helena Fernandes (Author) FMDUP; Rui Miranda Guedes (Author) FEUP; Maria Ascensão Lopes (Author) FEUP

Journal

Title: Polymer Testing

Vol. 86

Pages: 1-12

ISSN: 0142-9418

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 4 Citations

Other information

Authenticus ID: P-00S-097

DOI: 10.1016/j.polymertesting.2020.106507

Abstract (EN): Driven by the market demand, several synthetic and natural grafts have been proposed during the last years for tendons regeneration. The synthetic grafts, which present a better mechanical performance than the natural ones, usually fail due to the lack of biocompatibility and bioactivity. Thus, chitosan (Cs) was immobilized on polypropylene (PP) surface, previously activated by plasma treatment, in order to improve the fibroblasts' adhesion and proliferation on it. The Orange II dye method and FTIR-ATR analysis proved the successful Cs immobilization on the PP surface. It was observed by SEM and optical profilometry analysis that the Cs concentration increase leads to a surface roughness (R-a value) increase, as well as to water contact angle (C.A.) decrease at least until 2% (w/V) of Cs. Using the 2% (V/V) Cs solution concentration, according to SEM analysis and resazurin assay, the developed functionalization was well succeeded in improving fibroblasts adhesion and proliferation on PP substrates surface over 7 days of culture.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12