In vitro antimicrobial activity and biocompatibility of propolis containing nanohydroxyapatite

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Liliana Grenho (Author) FEUP; Barros, J (Author) Other; Ferreira, C (Author) Other; Santos, VR (Author) Other; Fernando Mendes Monteiro (Author) FEUP; Ferraz, MP (Author) Other; Cortes, ME (Author) Other

Journal

Title: Biomedical Materials (Bristol)

Vol. 10 No. 5

Final page: 025004

ISSN: 1748-6041

Publisher: Institute of Physics Publishing

Indexing

ISI Web of Knowledge - 31 Citations

Scopus - 33 Citations

Other information

Authenticus ID: P-00A-D48

DOI: 10.1088/1748-6041/10/2/025004

Abstract (EN): The high number of biomaterial associated infections demands new strategies to prevent this problem. In this study the suitability of nanohydroxyapatite (nanoHA)-based surfaces containing two Brazilian extracts of propolis (green and red ones) to prevent bacterial growth and biofilm formation, as well as its non-cytotoxic nature, was investigated. Optical density, colony forming units and MTT reduction assay were used to assess the materials' antibacterial activity against planktonic and sessile growth of Staphylococcus aureus. NanoHA matrix was able to absorb both types of propolis and the obtained results revealed the antibacterial effectiveness of the novel materials expressed as the reduction of bacterial growth and biofilm formation ability. Additionally, cell culture tests showed the growth of fibroblasts with high metabolic activity and without membrane damage. Therefore, these nanoHA-based surfaces containing natural products deriving from bees may be a promising bioactive biomaterial to be further studied with the aim of application to orthopaedic or dental devices.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8