Preparative separation of ketoprofen enantiomers: Choice of mobile phase composition and measurement of competitive adsorption isotherms

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Ribeiro, AE (Author) Other; Graca, NS (Author) Other; Pais, LS (Author) Other; Alírio Rodrigues (Author) FEUP

Journal

Title: Separation and Purification Technology

Vol. 61 No. 3

Pages: 375-383

ISSN: 1383-5866

Publisher: Elsevier

Indexing

ISI Proceedings

ISI Web of Knowledge - 16 Citations

ISI Web of Science

Scopus - 16 Citations

COMPENDEX

Scientific classification

FOS: Engineering and technology

CORDIS: Technological sciences > Engineering > Chemical engineering

Other information

Authenticus ID: P-003-XS3

DOI: 10.1016/j.seppur.2007.11.010

Abstract (EN): The present work intends to investigate how mobile phase composition influences the adsorption behavior of ketoprofen enantiomers (a nonsteroidal anti-inflainmatory drug) on an amylose-based chiral stationary phase (Chiralpak AD). Three mobile phase compositions were studied: the usual 20% ethanol/80% n-hexane mixture and two pure mobile phases; methanol and ethanol, Pulse and breakthrough experiments under preparative conditions were carried out in order to measure and test adsorption isotherms. The results obtained show that, for preparative separations, pure ethanol is a better mobile phase than the usual 20% ethanol/80% n-hexane mixture: it allows higher solubility of the racemate, lower retention times, and also a higher selectivity at high enantiomer concentrations. These are aspects of crucial importance when the final goal is to achieve high productivity preparative separations, as it is shown for the simulated moving bed (SMB) operation.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9