Volatilomics Reveals Potential Biomarkers for Identification of Renal Cell Carcinoma: An In Vitro Approach

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Amaro, F (Author) Other; Pinto, J (Author) Other; Rocha, S (Author) Other; Araujo, AM (Author) Other; Miranda Goncalves, V (Author) Other; Carmen Jeronimo (Author) ICBAS; henrique, rmf (Author) ICBAS; Maria de Lourdes Bastos (Author) FFUP; Carvalho, M (Author) Other; de Pinho, PG (Author) FCUP

Journal

Title: Metabolites

Vol. 10

Final page: 174

ISSN: 2218-1989

Publisher: MDPI

Indexing

ISI Web of Knowledge - 9 Citations

Scopus - 9 Citations

Other information

Authenticus ID: P-00S-1MJ

DOI: 10.3390/metabo10050174

Abstract (EN): The identification of noninvasive biomarkers able to detect renal cell carcinoma (RCC) at an early stage remains an unmet clinical need. The recognition that altered metabolism is a core hallmark of cancer boosted metabolomic studies focused in the search for cancer biomarkers. The present work aims to evaluate the performance of the volatile metabolites present in the extracellular medium to discriminate RCC cell lines with distinct histological subtypes (clear cell and papillary) and metastatic potential from non-tumorigenic renal cells. Hence, volatile organic compounds (VOCs) and volatile carbonyl compounds (VCCs) were extracted by headspace solid-phase microextraction (HS-SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). Multivariate and univariate analysis unveiled a panel of metabolites responsible for the separation between groups, mostly belonging to ketones, alcohols, alkanes and aldehydes classes. Some metabolites were found similarly altered for all RCC cell lines compared to non-tumorigenic cells, namely 2-ethylhexanol, tetradecane, formaldehyde, acetone (increased) and cyclohexanone and acetaldehyde (decreased). Furthermore, significantly altered levels of cyclohexanol, decanal, decane, dodecane and 4-methylbenzaldehyde were observed in all metastatic RCC cell lines when compared with the non-metastatic ones. Moreover, some alterations in the volatile composition were also observed between RCC histological subtypes. Overall, our results demonstrate the potential of volatile profiling for identification of noninvasive candidate biomarkers for early RCC diagnosis.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 19