RIBE at an inter-organismic level: A study on genotoxic effects in Daphnia magna exposed to waterborne uranium and a uranium mine effluent

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Ruth Pereira (Author) FCUP; Reis, P. (Author) Other; Lourenço, J. (Author) Other; Carvalho, F.P. (Author) Other; Oliveira, J. (Author) Other; Malta, M. (Author) Other; Mendo, S. (Author) Other

Journal

Title: Aquatic Toxicology

Vol. 198

Pages: 206-214

ISSN: 0166-445X

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 7 Citations

Other information

Authenticus ID: P-00N-S18

DOI: 10.1016/j.aquatox.2018.03.007

Abstract (EN): The induction of RIBE (Radiation Induced Bystander Effect) is a non-target effect of low radiation doses that has already been verified at an inter-organismic level in fish and small mammals. Although the theoretical impact in the field of environmental risk assessment (ERA) is possible, there is a gap of knowledge regarding this phenomenon in invertebrate groups and following environmentally relevant exposures. To understand if RIBE should be considered for ERA of radionuclide-rich wastewaters, we exposed Daphnia magna (< 24 h and 5d old) to a 2% diluted uranium mine effluent for 48 h, and to a matching dose of waterborne uranium (55.3 mu g L-1). Then the exposed organisms were placed (24 and 48 h) in a clean medium together with non-exposed neonates. The DNA damage observed for the non-exposed organisms was statistically significant after the 24 h cohabitation for both uranium (neonates p = 0.002; 5 d-old daphnids p = < 0.001) and uranium mine effluent exposure (only for neonates p = 0.042). After 48 h cohabitation significant results were obtained only for uranium exposure (neonates p = 0.017; 5 d-old daphnids p = 0.013). Although there may be some variability associated to age and exposure duration, the significant DNA damage detected in non-exposed organisms clearly reveals the occurrence of RIBE in D. magna. The data obtained and here presented are a valuable contribution for the discussion about the relevance of RISE for environmental risk assessment.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9