Evaluation of inducible promoters on the secretion of a ZZ-proinsulin fusion protein in Escherichia coli

• Type: Article in International Scientific Journal
• Date: 2003

Authors

Mergulhao, FJM (Author) Other; Monteiro, GA (Author) Other; Larsson, G (Author) Other; Bostrom, M (Author) Other; Farewell, A (Author) Other; Nystrom, T (Author) Other; Cabral, JMS (Author) Other; Taipa, MA (Author) Other

Journal

Title: Biotechnology and Applied Biochemistry

Vol. 38

Pages: 87-93

ISSN: 0885-4513

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 14 Citations

Scopus - 15 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-000-FKZ

DOI: 10.1042/ba20030043

Abstract (EN): Four inducible promoters, uspA, uspB, IacUV5 and malK, were evaluated in the expression of the fusion protein ZZ-proinsulin by Escherichia coli. The aim was to select for their effects on the most appropriate expression system (promoter and culture medium) for secretion of ZZ-proinsulin to the periplasmic space and culture medium. All the expression vectors contained the RNase III cleavage site to ensure that the mRNA translation rate remained independent of 5'-untranslated regions thus making promoter strength comparisons more accurate. The highest ZZ-proinsulin secretion yields were 6.2 mg/g of dry cell weight in the periplasmic space and 2.6 mg/g of dry cell weight in the culture medium using the malK promoter. It was also demonstrated that the use of M9 minimal medium favours secretion.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7