Bioactive Marine Natural Products

Code:

RM10

Acronym:

PNMB

Keywords
Classification	Keyword
OFICIAL	Marine Sciences

Instance: 2018/2019 - 1S

Active?	Yes
Responsible unit:	Chemistry
Course/CS Responsible:	Master Degree in Marine Sciences - Marine Resources

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
MCMRM	5	Oficial Plan 2018	1	-	5	50	135

Teaching language

Suitable for English-speaking students

Objectives

This curricular unit aims at giving students a broad knowledge of several classes of secondary metabolites (commonly known as Natural Products) produced by marine macro- and microorganisms as well as their potential applications in pharmaceutical, cosmeceutical and food industries.

 

Additionally, this curricular unit also intends to give the students an opportunity to learn the methods of extraction, purification and structure elucidation as well as some bioassays to evaluate some biological activities of marine natural products.

It is expected that, as a result of this Curricular Unit, the students are able acquire a capacity to distinguish, identify and evaluate various classes of bioactive compounds produced by marine organisms for industrial application.

Learning outcomes and competences

The curriculum unit (CU) deals with an attempt to present a recent evolution of Chemistry of Marine Natural Products in the field of bioprospection, extraction, isolation, structure elucidation as well as their potential applications in pharmaceutical, cosmetic and food industries. The knowledge acquired from this CU can contribute to a better capacity to exploit the richness of the marine resources which is in agreement with the objectives of the Master’s program. The theoretical and practical program proposed in the syllabus support all the objectives described in the “Learning Objectives”. Furthermore, the incorporation of different types of practical works will allow students to acquire additional knowledge for the proposed expertise, especially to “differentiate, identify and evaluate” mentioned in “Learning Objectives”. The continuous evaluation of the knowledge acquired in the CU is destined to promote a progressive learning, especially taking into account that the students in this master’s program have diverse backgrounds.

Working method

Presencial

Program

Theoretical:

Introduction

Definition of Secondary Metabolites

Classification of Secondary Metabolites based on chemical structure Classification of Secondary Metabolites based on biosynthetic origin

Methods of extraction of marine natural products from marine invertebrates and marine-derived fungi Methods of isolation and purification of marine natural products

Methods of identification of marine natural products-1D and 2D, high resolution mass spectroscopy and X-ray crystallography E.g. of Marine Natural Products with antibacterial, antifungal, anticancer and antilipidemic activities and used for drugs, cosmetics and food supplements

 

Practical:

Isolation and purification of Marine Natural Products by column and Thin Layer Chromatography

Analysis of 1D and 2D NMR spectra, HRMS spectra and interpretation of ORTEP diagram of X-ray crystallography

In vitro Bioassays for antibacterial, antifungal, anticancer and antilipidemic activity of compounds isolated from Marine Organisms

Mandatory literature

E. Ibañez, F. Rindi, M. Hayes; Marine Bioactive Compounds: Source, Characterization and Application, Maria Hyes , 2014. ISBN: 13: 978- 1489986108
Torger Bǿrrensen, Catherine Boyen, Alan Dobson, Manfred Höfle, Adriana Ianora, Marcel Jaspars, AnakeKijjoa, Jan Olafsen, Joel Querellou, Gorges Rigos, René Wijffels ; Marine Biotechnology: A new vision and strategy for Europe. Marine Board-ESF Position Paper 15, 2010

Complementary Bibliography

. Amy L. Lane and Bradley S. Moore ; A sea of biosynthesis: marine natural products meet the molecular Age, Nat. Prod. Rep. 28, 411-428., 2011

Teaching methods and learning activities

Power Point Presentations will be used for lectures which allows to promote discussion and interaction with students as well as interaction of knowledge and critical thinking of students. The practical classes will be destined for a laboratorial work on isolation and purification of the compounds from the crude extracts of marine organism, interpretation of various sets of NMR and HRMS spectra as well as the ORTEP diagram of the X-ray crystallography to solve the structure of marine natural products from different sources, as well as the biological activity evaluations, with emphasis in vitro, of the compounds isolated from Marine Organisms. Evaluation of the students will be done through final examination

keywords

Physical sciences > Chemistry > Organic chemistry
Physical sciences > Chemistry > Applied chemistry > Pharmaceutical chemistry
Natural sciences
Technological sciences > Technology > Biotecnology

Evaluation Type

Distributed evaluation without final exam

Assessment Components

Designation	Weight (%)
Participação presencial	100,00
Total:	100,00

Amount of time allocated to each course unit

Designation	Time (hours)
Frequência das aulas	30,00
Trabalho laboratorial	20,00
Total:	50,00

Eligibility for exams

75% attendance.

Calculation formula of final grade

Continuous evaluation: 70%
Report: 30%