Project: POCI-01-0145-FEDER-016732

Project name:	ADIPONEO - Study of the immune function of adipose tissue in Neospora caninum infected hosts
Project code:	POCI-01-0145-FEDER-016732
Main Objective:	Reforçar a investigação, o desenvolvimento tecnológico e a inovação
Intervention Region:	Norte
Proposing institution/Lead promoter/Coordinating entity:	Instituto de Ciências Biomédicas de Abel Salazar
Partner(s)/Co-promoter(s)/Participating institution(s):	Instituto de Biologia Molecular e Celular; Instituto de Investigação e Inovação em Saúde; Unidade Multidisciplinar de Investigação Biomédica
Date of approval:	2016-03-23
Start date:	2016-06-01
Completion date:	2020-08-31

Eligible Cost of the Project

Total Eligible Cost:	184.610,00 EUR
Eligible Cost in the University of Porto:	158.810,00 EUR
Instituto de Ciências Biomédicas de Abel Salazar:	158.810,00 EUR

Total Financial Support

União Europeia - FEDER:	156.918,50 EUR
Orçamento de Estado:	27.691,50 EUR

Financial Support to the University of Porto

Total of the University of Porto:	158.810,00 EUR
União Europeia \| União Europeia - FEDER \| Instituto de Ciências Biomédicas de Abel Salazar:	134.988,50 EUR
Nacional/Regional \| Orçamento de Estado \| Instituto de Ciências Biomédicas de Abel Salazar:	23.821,50 EUR

Objectives, activities and expected/achieved results

Objetivos e Atividades Neospora caninum is an apicomplexa parasite that in cattle assumes particular importance as it is responsible for abortions reported in many countries, including Portugal. Mice acutely infected with N. caninum presented marked immune cell alterations in adipose tissue that were still detectable in chronically infected animals. In this project, we intend to further explore the role of adipose tissue cell populations in the outcome of this parasitic infection, not only in the murine model but, more importantly, in the bovine host. Particularly, one goal is to determine which immune cell populations are present in cow's adipose tissue and how are they affected in cattle naturally infected with N. caninum. In order to achieve this goal, adipose tissue (subcutaneous and visceral) will be recovered from cows seropositive to Neospora caninum. Characterization of the immune cell populations of adipose tissue will then be assessed by flow cytometry and compared with seronegative animals.
Resultados Esperados/Atingidos We developed multicolour flow cytometry panels that enable us to describe and characterize diverse lymphocyte and myeloid populations from adipose tissue of Holstein-Friesian cows.Regarding lymphocyte populations,our panel enable identification of γδ T cells,CD4+CD3+non-γδ T cells,CD8+CD3+non-γδ T cells and NK cells in mesenteric and subcutaneous adipose tissue of bovines(MAT and SAT, respectively),with T cells presenting predominantly a memory-like phenotype. Another panel allowed identification of macrophages,neutrophils and mast cells in bovine MAT and SAT. We found that bovines seropositive to N.caninum(naturally exposed to the parasite)presented higher frequencies of macrophages in SAT comparatively to seronegative ones.Contrastingly, a decreased frequency of a population CD45−with a CH138A+CD11b−MHC-II−phenotype was observed in MAT and SAT of the animals seropositive to N.caninum.

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