Sequential injection chromatographic determination of paracetamol, caffeine, and acetylsalicylic acid in pharmaceutical tablets

• Tipo: Artigo em Revista Científica Internacional
• Data: 2004

Autores

Satinsky, D (Autor) Outra; Neto, I (Autor) Outra; Solich, P (Autor) Outra; Sklenarova, H (Autor) Outra; Conceicao, M (Autor) FFUP; Montenegro, BSM (Autor) Outra; Araujo, AN (Autor) FFUP

Revista

Título: Journal of Separation Science

Vol. 27 Nº 7

Páginas: 529-536

ISSN: 1615-9306

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 66 Citações

ISI Web of Science

Scopus - 79 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

CORDIS: Ciências da Saúde

Outras Informações

ID Authenticus: P-000-AMY

DOI: 10.1002/jssc.200301644

Resumo (PT): In this contribution, a new separation method for simultaneous determination of paracetamol, caffeine, acetylsalicylic acid, and internal standard benzoic acid was developed based on a novel reversed-phase sequential injection chromatography (SIC) technique with UV detection. A Chromolith® Flash RP-18e, 25-4.6 mm column (Merck, Germany) and a FIAlab® 3000 system (USA) with an 8-port selection valve and a 5 mL syringe were used for sequential injection chromatographic separations in our study. The mobile phase used was acetonitrile-(0.01 M) phosphate buffer (10 : 90, v/v) pH 4.05, flow rate 0.6 mL min-1. UV detection was at 210 and 230 nm. The validation parameters showed good results: linearity (r >0.999) for all compounds, detection limits in the range 0.3-0.8 g mL-1, repeatability (RSD) of peak heights between runs in the range 1.10-4.30% at three concentration levels and intra-day repeatability of the retention times in the range 0.28-0.43%. The analysis time was < 6 min. The method was found to be applicable for the routine analysis of the active compounds paracetamol, caffeine, and acetylsalicylic acid in pharmaceutical tablets. <br> <br> Keywords Sequential injection analysis (SIA) • Sequential injection chromatography (SIC) • Monolithic columns • Paracetamol • Caffeine • Acetylsalicylic acid <br> <a target="_blank" href="http://www3.interscience.wiley.com/journal/107642973/abstract"> texto integral</a> <br> <br>

Abstract (EN): In this contribution, a new separation method for simultaneous determination of paracetamol, caffeine, acetylsalicylic acid, and internal standard benzoic acid was developed based on a novel reversed-phase sequential injection chromatography (SIC) technique with UV detection. A Chromolith(R) Flash RP-18e, 25-4.6 mm column (Merck, Germany) and a FIAlab(R) 3000 system (USA) with an 8-port selection valve and a 5 mL syringe were used for sequential injection chromatographic separations in our study. The mobile phase used was acetonitrile-(0.01 M) phosphate buffer (10: 90, v/v) pH 4.05, flow rate 0.6 mL min(-1). UV detection was at 210 and 230 nm. The validation parameters showed good results: linearity (r>0.999) for all compounds, detection limits in the range 0.3-0.8 mug mL(-1), repeatability (RSD) of peak heights between runs in the range 1.10-4.30% at three concentration levels and intra-day repeatability of the retention times in the range 0.28-0.43%. The analysis time was <6 min. The method was found to be applicable for the routine analysis of the active compounds paracetamol, caffeine, and acetylsalicylic acid in pharmaceutical tablets.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8