Sequential injection chromatographic determination of paracetamol, caffeine, and acetylsalicylic acid in pharmaceutical tablets

• Type: Article in International Scientific Journal
• Date: 2004

Authors

Satinsky, D (Author) Other; Neto, I (Author) Other; Solich, P (Author) Other; Sklenarova, H (Author) Other; Conceicao, M (Author) FFUP; Montenegro, BSM (Author) Other; Araujo, AN (Author) FFUP

Journal

Title: Journal of Separation Science

Vol. 27 No. 7

Pages: 529-536

ISSN: 1615-9306

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 66 Citations

ISI Web of Science

Scopus - 79 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-000-AMY

DOI: 10.1002/jssc.200301644

Resumo (PT): In this contribution, a new separation method for simultaneous determination of paracetamol, caffeine, acetylsalicylic acid, and internal standard benzoic acid was developed based on a novel reversed-phase sequential injection chromatography (SIC) technique with UV detection. A Chromolith® Flash RP-18e, 25-4.6 mm column (Merck, Germany) and a FIAlab® 3000 system (USA) with an 8-port selection valve and a 5 mL syringe were used for sequential injection chromatographic separations in our study. The mobile phase used was acetonitrile-(0.01 M) phosphate buffer (10 : 90, v/v) pH 4.05, flow rate 0.6 mL min-1. UV detection was at 210 and 230 nm. The validation parameters showed good results: linearity (r >0.999) for all compounds, detection limits in the range 0.3-0.8 g mL-1, repeatability (RSD) of peak heights between runs in the range 1.10-4.30% at three concentration levels and intra-day repeatability of the retention times in the range 0.28-0.43%. The analysis time was < 6 min. The method was found to be applicable for the routine analysis of the active compounds paracetamol, caffeine, and acetylsalicylic acid in pharmaceutical tablets. <br> <br> Keywords Sequential injection analysis (SIA) • Sequential injection chromatography (SIC) • Monolithic columns • Paracetamol • Caffeine • Acetylsalicylic acid <br> <a target="_blank" href="http://www3.interscience.wiley.com/journal/107642973/abstract"> texto integral</a> <br> <br>

Abstract (EN): In this contribution, a new separation method for simultaneous determination of paracetamol, caffeine, acetylsalicylic acid, and internal standard benzoic acid was developed based on a novel reversed-phase sequential injection chromatography (SIC) technique with UV detection. A Chromolith(R) Flash RP-18e, 25-4.6 mm column (Merck, Germany) and a FIAlab(R) 3000 system (USA) with an 8-port selection valve and a 5 mL syringe were used for sequential injection chromatographic separations in our study. The mobile phase used was acetonitrile-(0.01 M) phosphate buffer (10: 90, v/v) pH 4.05, flow rate 0.6 mL min(-1). UV detection was at 210 and 230 nm. The validation parameters showed good results: linearity (r>0.999) for all compounds, detection limits in the range 0.3-0.8 mug mL(-1), repeatability (RSD) of peak heights between runs in the range 1.10-4.30% at three concentration levels and intra-day repeatability of the retention times in the range 0.28-0.43%. The analysis time was <6 min. The method was found to be applicable for the routine analysis of the active compounds paracetamol, caffeine, and acetylsalicylic acid in pharmaceutical tablets.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8