Sampling strategies exploiting multi-pumping flow systems

• Tipo: Artigo em Revista Científica Internacional
• Data: 2003

Autores

Prior, JAV (Autor) FFUP; Santos, JLM (Autor) FFUP; Lima, JLFC (Autor) FFUP

Revista

Título: Analytical and Bioanalytical Chemistry

Vol. 375 Nº 8

Páginas: 1234-1239

ISSN: 1618-2642

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 10 Citações

ISI Web of Science

Scopus - 12 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

CORDIS: Ciências da Saúde

Outras Informações

ID Authenticus: P-000-H3T

DOI: 10.1007/s00216-003-1813-5

Resumo (PT): In this work new strategies were exploited to implement multi-pumping flow systems relying on the utilisation of multiple devices that act simultaneously as sample-insertion, reagent-introduction, and solution-propelling units. The solenoid micro-pumps that were initially used as the only active elements of multi-pumping systems, and which were able to produce pulses of 3 to 25 wL, were replaced by syringe pumps with the aim of producing pulses between 1 and 4 wL. The performance of the developed flow system was assessed by using distinct sample-insertion strategies like single sample volume, merging zones, and binary sampling in the spectrophotometric determination of isoniazid in pharmaceutical formulations upon reaction with 1,2-naphthoquinone-4-sulfonate, in alkaline medium. The results obtained showed that enhanced sample/reagent mixing could be obtained with binary sampling and by using a 1 wL per step pump, even in limited dispersion conditions. Moreover, syringe pumps produce very reproducible flowing streams and are easily manipulated and controlled by a computer program, which is greatly simplified since they are the only active manifold component. Linear calibration plots up to 18.0 wg mLm1, with a relative standard deviation of less than 1.48% (n=10) and a throughput of about 20 samples per hour, were obtained. <br> <br> Keywords Multi-pumping flow systems · Binary sampling · Isoniazid · 1,2-Naphthoquinone-4-sulfonate Pharmaceuticals <br> <a target="_blank" href="http://www.springerlink.com/content/elefvm21147q03ky/?p=e57cc288bfa74b3e85df6675a241e93d&pi=42 "> Texto integral </a> <br> <br>

Abstract (EN): In this work new strategies were exploited to implement multi-pumping flow systems relying on the utilisation of multiple devices that act simultaneously as sample-insertion, reagent-introduction, and solution-propelling units. The solenoid micro-pumps that were initially used as the only active elements of multi-pumping systems, and which were able to produce pulses of 3 to 25 muL, were replaced by syringe pumps with the aim of producing pulses between I and 4 muL. The performance of the developed flow system was assessed by using distinct sample-insertion strategies like single sample volume, merging zones, and binary sampling in the spectrophotometric determination of isoniazid in pharmaceutical formulations upon reaction with 1,2-naphthoquinone-4-sulfonate, in alkaline medium. The results obtained showed that enhanced sample/reagent mixing could be obtained with binary sampling and by using a 1 muL per step pump, even in limited dispersion conditions. Moreover, syringe pumps produce very reproducible flowing streams and are easily manipulated and controlled by a computer program, which is greatly simplified since they are the only active manifold component. Linear calibration plots up to 18.0 mug mL(-1), with a relative standard deviation of less than 1.48% (n=10) and a throughput of about 20 samples per hour, were obtained.

Idioma: Inglês

Tipo (Avaliação Docente): Científica