LPS-Induced Mortality in Zebrafish: Preliminary Characterisation of Common Fish Pathogens

• Tipo: Artigo em Revista Científica Internacional
• Data: 2023

Autores

Santos, RA (Autor) Outra; Cardoso, C (Autor) Outra; Pedrosa, N (Autor) Outra; Gonçalves, G (Autor) Outra; Matinha-Cardoso, J (Autor) Outra; Coutinho, F (Autor) Outra; Carvalho, AP (Autor) FCUP; Paula Tamagnini (Autor) FCUP; Oliva Teles, A (Autor) FCUP; Oliveira, P (Autor) FCUP; Claudia Alexandra dos Reis Serra (Autor) FCUP

Revista

Título: Microorganisms

Vol. 11

Página Final: 2205

Editora: MDPI

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00Y-YW4

DOI: 10.3390/microorganisms11092205

Abstract (EN): Disease outbreaks are a common problem in aquaculture, with serious economic consequences to the sector. Some of the most important bacterial diseases affecting aquaculture are caused by Gram-negative bacteria including Vibrio spp. (vibriosis), Photobacterium damselae (photobacteriosis), Aeromonas spp. (furunculosis; haemorrhagic septicaemia) or Tenacibaculum maritimum (tenacibaculosis). Lipopolysaccharides (LPS) are important components of the outer membrane of Gram-negative bacteria and have been linked to strong immunogenic responses in terrestrial vertebrates, playing a role in disease development. To evaluate LPS effects in fish, we used a hot-phenol procedure to extract LPS from common fish pathogens. A. hydrophila, V. harveyi, T. maritimum and P. damselae purified LPS were tested at different concentrations (50, 100, 250 and 500 mu g mL-1) at 3 days post-fertilisation (dpf) Danio rerio larvae, for 5 days. While P. damselae LPS did not cause any mortality under all concentrations tested, A. hydrophila LPS induced 15.5% and V. harveyi LPS induced 58.3% of zebrafish larvae mortality at 500 mu g mL-1. LPS from T. maritimum was revealed to be the deadliest, with a zebrafish larvae mortality percentage of 80.6%. Analysis of LPS separated by gel electrophoresis revealed differences in the overall LPS structure between the bacterial species analysed that might be the basis for the different mortalities observed.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13