Screening of Natural Molecules as Adjuvants to Topical Antibiotics to Treat Staphylococcus aureus from Diabetic Foot Ulcer Infections

• Tipo: Artigo em Revista Científica Internacional
• Data: 2022

Autores

Oliveira, D (Autor) Outra; Anabela Borges (Autor) FEUP; Saavedra, MJ (Autor) Outra; Fernanda Borges (Autor) FCUP; Simoes, M (Autor) Outra; Simões M (Autor) FEUP

Revista

Título: Antibiotics

Vol. 11

Página Final: 620

Editora: MDPI

Indexação

ISI Web of Knowledge - 5 Citações

Scopus

Projetos Associados

ALiCE - ALiCE - Laboratório Associado em Engenharia Química

LEPABE - Financiamento Plurianual 2020-2023 da Unidade de I&D LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Biocide_for_Biofilm - Desenvolvimento de novas soluções biocidas para o controlo efetivo de biofilmes

GermIrrad - Sistema automatizado de irradiação germicida

Healthy Waters - Healthy Waters - Identification, Elimination, Social Awareness and Education of Water Chemical and Biological Micropollutants with Health and Environmental Implications

Outras Informações

ID Authenticus: P-00W-FMX

DOI: 10.3390/antibiotics11050620

Abstract (EN): Diabetic foot ulcers (DFUs) are a common result of a complex secondary complication of diabetes mellitus. More than half of DFUs become infected due to frequent colonization with Staphylococcus aureus. The use of topical antibiotics is proposed, especially in combination with natural adjuvants, to minimize the negative impacts caused by generalized use of systemic antibiotics. In this study, 13 different phytochemicals-namely chalcone, juglone, cinnamic acid, trigonelline, Furvina-and four nitrovinylfuran derivatives-guaiazulene, alpha-bisabolol, farnesol and nerolidol-were selected to be tested as antibiotic enhancers. After minimum inhibitory and bactericidal concentration (MIC and MBC) determination of each molecule against different strains of S. aureus, including clinical isolates from diabetic foot wounds (CECT 976, Xu212, SA 1199B, RN4220, MJMC102, MJMC109, MJMC110 and MJMC111), their potentiation effects on the antibiotics fusidic acid, mupirocin, gentamicin, oxacillin and methicillin were evaluated through the disc diffusion method. Farnesol at sub-MIC was able to restore the activity of methicillin and oxacillin on the MJMC102 and MJMC111 strains, as well as two MRSA clinical isolates, and potentiated the effect of the remaining antibiotics. The results obtained demonstrate the great potential for the topical application of phytochemicals and derivatives as antibiotic resistance modifier agents to combat multidrug resistance in bacterial wound infections.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 17