Fast screening procedure for antibiotics in wastewaters by direct HPLC-DAD analysis

• Tipo: Artigo em Revista Científica Internacional
• Data: 2008

Autores

Salome Teixeira (Autor) Outra; Cristina Delerue Matos (Autor) Outra; Arminda Alves (Autor) FEUP; Lucia Santos (Autor) FEUP

Revista

Título: Journal of Separation Science

Vol. 31 Nº 3

Páginas: 2924-2931

ISSN: 1615-9306

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 24 Citações

ISI Web of Science

Scopus - 26 Citações

COMPENDEX

Classificação Científica

FOS: Ciências exactas e naturais > Química

CORDIS: Ciências Tecnológicas

Outras Informações

ID Authenticus: P-003-WMR

DOI: 10.1002/jssc.200800229

Abstract (EN): Growing concern about the contamination of wastewaters by antibiotics demands fast but sensitive analytical methodologies, for the screening of a large number of samples. The purpose of this work was to develop a simple methodology, using direct injection of the samples, by HPLC with diode array detection (DAD), for a multiresidue analysis of five antibiotics of different classes. Wastewater from an urban water treatment plant was selected as a model to study possible coelution of interfering compounds. The linearity interval ranged from 40 to 400 mu g/L for amoxicillin (Amox), metronidazole (Metro), cefazolin (Cefa), and chloramphenicol (Chloram) and from 20 to 200 mu g/L for sulfamethoxazole (Sulfa), with LODs lower than 14 mu g/L. Repeatability, expressed by the CV of six repeated injections, ranged from 1 to 8%, while the intermediate precision varied between 2 and 11%. The recovery ranged from 90 to 109%. This method enables the fast screening of a large number of samples, with an expanded uncertainty in the 1 - 22% range. The advantage of the proposed method is to significantly reduce the number of samples to be analyzed by more complex methods.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8