Fast screening procedure for antibiotics in wastewaters by direct HPLC-DAD analysis

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Salome Teixeira (Author) Other; Cristina Delerue Matos (Author) Other; Arminda Alves (Author) FEUP; Lucia Santos (Author) FEUP

Journal

Title: Journal of Separation Science

Vol. 31 No. 3

Pages: 2924-2931

ISSN: 1615-9306

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 24 Citations

ISI Web of Science

Scopus - 26 Citations

COMPENDEX

Scientific classification

FOS: Natural sciences > Chemical sciences

CORDIS: Technological sciences

Other information

Authenticus ID: P-003-WMR

DOI: 10.1002/jssc.200800229

Abstract (EN): Growing concern about the contamination of wastewaters by antibiotics demands fast but sensitive analytical methodologies, for the screening of a large number of samples. The purpose of this work was to develop a simple methodology, using direct injection of the samples, by HPLC with diode array detection (DAD), for a multiresidue analysis of five antibiotics of different classes. Wastewater from an urban water treatment plant was selected as a model to study possible coelution of interfering compounds. The linearity interval ranged from 40 to 400 mu g/L for amoxicillin (Amox), metronidazole (Metro), cefazolin (Cefa), and chloramphenicol (Chloram) and from 20 to 200 mu g/L for sulfamethoxazole (Sulfa), with LODs lower than 14 mu g/L. Repeatability, expressed by the CV of six repeated injections, ranged from 1 to 8%, while the intermediate precision varied between 2 and 11%. The recovery ranged from 90 to 109%. This method enables the fast screening of a large number of samples, with an expanded uncertainty in the 1 - 22% range. The advantage of the proposed method is to significantly reduce the number of samples to be analyzed by more complex methods.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8