Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: in vitro mineralization and biological evaluation

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

Filipa Castro (Autor) FEUP; António Ferreira (Autor) FEUP; Viviana P. Ribeiro (Autor) Outra; Ana L. Oliveira (Autor) Outra; Rui L. Reis (Autor) Outra; José A. Teixeira (Autor) Outra; Fernando Rocha (Autor) FEUP

Revista

Título: Materials Research Express

Vol. 3 Nº 7

Página Final: 075404

Editora: Institute of Physics Publishing

Indexação

ISI Web of Knowledge - 10 Citações

Scopus - 7 Citações

INSPEC

Classificação Científica

CORDIS: Ciências Tecnológicas > Engenharia > Engenharia química

FOS: Ciências da engenharia e tecnologias > Engenharia química

Projetos Associados

IF/01087/2014/CP1249/CT0001 - Projeto de investigação exploratória

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Outras Informações

ID Authenticus: P-00K-R6C

DOI: 10.1088/2053-1591/3/7/075404

Abstract (EN): This work reports the biological evaluation of nanosized hydroxyapatite (HAp) previously synthesized by continuous-flow precipitation in a scaled-up meso oscillatory flow reactor (meso-OFR). Physicochemical characterization of the synthesized HAp suggests high surface reactivity namely because of its high specific surface area and low crystallinity. On the other hand, in vitro biomineralization assays demonstrated the apatite-forming activity of the prepared HAp and their higher surface reactivity when compared to a commercial HAp. Furthermore, human osteoblastic-like (Saos-2) cells culture evidenced that the synthesized HAp stimulated cell proliferation, especially when applied at lower concentrations (30 and 50 mu g ml(-1)), although its cellular uptake behavior. Therefore, the prepared HAp shows immense potential as biomedical material, as well as drug and gene delivery vehicle. The results are also very promising regarding further scaling up of the process, as the designed methodology allow for the preparation in a continuous mode of nanosized HAp with controlled physico-chemical properties.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13