Inhibition of norovirus replication by the nucleoside analogue 2 '-C-methylcytidine

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Rocha Pereira, J (Autor) Outra; Jochmans, D (Autor) Outra; Dallmeier, K (Autor) Outra; Leyssen, P (Autor) Outra; Cunha, R (Autor) Outra; Costa, I (Autor) Outra; Nascimento, MSJ (Autor) FFUP; Neyts, J (Autor) Outra

Revista

Título: Biochemical and Biophysical Research Communications

Vol. 427

Páginas: 796-800

ISSN: 0006-291X

Editora: Elsevier

Indexação

ISI Web of Knowledge - 33 Citações

Scopus - 12 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-002-3G2

DOI: 10.1016/j.bbrc.2012.10.003

Abstract (EN): We here report on the activity of 2 '-C-methylcytidine (2CMC) [a nucleoside polymerase inhibitor of the hepatitis C virus (HCV)] on the in vitro replication of (murine) norovirus (MNV). 2CMC inhibits (i) virus-induced CPE formation, (ii) viral RNA synthesis and (iii) infectious progeny formation with EC50 values of similar to 2 mu M. 2CMC acts at a time-point that coincides with the onset of viral RNA synthesis. Even following 30 passages of selective pressure no MNV-resistant virus was selected, which is in line with the high barrier to resistance of the nucleoside analogue for HCV. When combined with the broad-spectrum RNA virus inhibitor ribavirin, a marked antagonistic activity was observed indicating that these molecules should not be combined for the treatment of norovirus infections. Our results suggest that 2 '-C-methyl nucleoside analogues should be further explored for the treatment and prophylaxis of norovirus infections.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5