Homologous STRs in the X and Y chromosomes: A contribution to understanding human sexual chromosomes evolution

• Tipo: Artigo em Livro de Atas de Conferência Internacional
• Data: 2000

Autores

Gusmao, L (Autor) FCUP; Sanchez Diz, P (Autor) Outra; Gonzalez Neira, A (Autor) Outra; Brion, M (Autor) Outra; Lareu, MV (Autor) Outra; Amorim, A (Autor) FCUP; Carracedo, A (Autor) Outra

Ata de Conferência Internacional

Título: PROGRESS IN FORENSIC GENETICS 8

Páginas: 64-66

18th Congress of the International-Society-for-Forensic-Haemogenetics (ISFH)

SAN FRANCISCO, CA, AUG 17-21, 1999

Indexação

ISI Web of Knowledge - 2 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Outras ciências médicas

Outras Informações

ID Authenticus: P-008-KM5

Abstract (EN): DYS391 and DYS393 primers, described in the Genome Data Base, were used to amplify DNA samples from both male and female origin. After the amplification of DNA from male blood samples it was possible to observe additional amplification products to the normal alleles for both systems when an annealing temperature of 55 degrees C was used. In addition, in the same size range a clear polymorphism was detected in females for both systems. For DYS391, a first screening in males on these minor amplified products allowed the identification of 5 different alleles. After the amplification of male DNA samples only single band patterns were observed while using the same conditions to amplify female DNA it was possible to observe the same polymorphism but with the presence of heterozygous patterns, as expected for a diploid genetic system. In order to improve the applicability of this system in forensic genetics, an alternative primer was designed to amplify the Y-chromosome specific STR.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 3