Mutated E-cadherin: Genomic and functional characterization in thyroid cells from the KAT family

• Tipo: Artigo em Revista Científica Internacional
• Data: 2004

Autores

Rocha, AS (Autor) Outra; De Wever, O (Autor) Outra; Moreira, S (Autor) Outra; Costa, MJ (Autor) Outra; Vandekerckhove, J (Autor) Outra; Mareel, M (Autor) Outra; Soares, P (Autor) FMUP

Revista

Título: Thyroid

Vol. 14

Páginas: 902-909

ISSN: 1050-7256

Editora: Mary Ann Liebert

Indexação

ISI Web of Knowledge - 7 Citações

Scopus - 7 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina clínica

Outras Informações

ID Authenticus: P-000-81H

DOI: 10.1089/thy.2004.14.902

Abstract (EN): Members of a family of thyroid cell lines (KAT) were analyzed because they expressed a higher molecular weight (135 kd) form of E-cadherin at their surface. We found that this aberrant E-cadherin is the result of a point mutation in the exon 9 donor splice site causing a skipping of exon 9 with consequent deletion of the corresponding aminoacids on E-cadherin protein. As a spin-off, we report that the various members of the KAT family share this mutation as well as the genetic background. Furthermore we found that this mutated protein leads to disturbed cell-cell adhesion although E-cadherin is still able to mediate the formation of the cadherin/catenin complex. We also demonstrate the presence of another cell-cell adhesion complex, formed by P-cadherin and the catenins. The latter is also not able to mediate cell-cell adhesion. Although these cells lack cell-cell adhesion they are not invasive without exogenous stimulus.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8