Improved clearance of Mycobacterium avium upon disruption of the inducible nitric oxide synthase gene

• Tipo: Artigo em Revista Científica Internacional
• Data: 1999

Autores

Gomes, MS (Autor) Outra; Florido, M (Autor) Outra; Pais, TF (Autor) Outra; Appelberg, R (Autor) ICBAS

Revista

Título: Journal of Immunology

Vol. 162

Páginas: 6734-6739

ISSN: 0022-1767

Editora: American Association of Immunologists

Indexação

ISI Web of Knowledge - 103 Citações

Scopus - 113 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

Outras Informações

ID Authenticus: P-001-47J

Abstract (EN): Mice genetically deficient in the inducible NO synthase gene (iNOS-/-) were used. to study the role played by NO during infection by Mycobacterium avium. iNOS-/- macrophages were equally able to restrict M, avium growth in vitro following stimulation by IFN-gamma and TNF-alpha as macrophages from wild-type mice. In vivo, the infection progressed at similar rates in wild-type-and NO-deficient mice during the first 2 mo of infection, but the latter mice were subsequently more efficient in clearing the mycobacteria than the former. The increased resistance of iNOS-/- mice was associated with higher IFN-gamma levels in the serum and following in vitro restimulation of spleen cells with specific Ag, increased formation of granulomas and increased survival of CD4(+) T cells. We show that NO is not involved in the antimycobacterial mechanisms of M, avium-infected macrophages and, furthermore, that it exacerbates the infection by causing the suppression of the immune response to the pathogen.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6