Distinct modulation of alkaline phosphatase isoenzymes by 17 beta-estradiol and xanthohumol in breast cancer MCF-7 cells

• Tipo: Artigo em Revista Científica Internacional
• Data: 2007

Autores

Susana Guerreiro (Autor) Outra; Rosario Monteiro (Autor) FCNAUP; Maria Joao Martins (Autor) FMUP; Conceicao Calhau (Autor) FMUP; Isabel Azevedo (Autor) Outra; Raquel Soares (Autor) FMUP

Revista

Título: Clinical Biochemistry

Vol. 40

Páginas: 268-273

ISSN: 0009-9120

Editora: Elsevier

Indexação

ISI Web of Knowledge - 36 Citações

Scopus - 39 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Engenharia médica

Outras Informações

ID Authenticus: P-004-BY9

DOI: 10.1016/j.clinbiochem.2006.09.012

Abstract (EN): Objectives: To examine the effect of 17 beta-estradiol and xanthohumol in alkaline phosphatase (ALP) expression and activity in breast cancer MCF-7 cells. Design and methods: ALP isoenzytnes expression was evaluated by RT-PCR and Western blotting. ALP activity was measured by spectrophotometry. Cell proliferation and apoptosis were examined by MTT and immunostaining for K167 and TUNEL, respectively. Results: ALP isoenzymes expression and activity were decreased by 1 nM 17 beta-estradiol. Pure estrogenic antagonist (ICI 182,780) reversed 17 beta-estradiol-inhibiting effect in TNS-ALP expression. RNA and protein expression of IALP, but not TNS-ALP, was also decreased by incubation with 10 mu M xanthohumol (IC50) and was accompanied by a significant reduction in ALP activity. Treatment with 17 beta-estradiol enhanced cell proliferation and decreased apoptosis. Conversely, xanthohumol incubation inhibited cell viability and apoptosis. Conclusion: Estrogens and xanthohumol differently modulate ALP isoenzymes. ALP loss associated with increased cell proliferation. Modulation of this enzyme by 17 beta-estradiol and xanthohumol might provide therapeutic strategies against hormone-dependent breast cancer.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6