Application of statistical experimental methodology to optimize reactive dye decolourization by commercial laccase

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Ana P. M. Tavares (Autor) FEUP; Raquel O. Cristovão (Autor) FEUP; José M. Loureiro (Autor) FEUP; Rui A. R. Boaventura (Autor) FEUP; Eugénia A. Macedo (Autor) FEUP

Revista

Título: Journal of Hazardous Materials

Vol. 162

Páginas: 1255-1260

ISSN: 0304-3894

Editora: Elsevier

Indexação

ISI Web of Knowledge - 64 Citações

ISI Web of Science

Scopus - 79 Citações

INSPEC

COMPENDEX

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Outras ciências da engenharia e tecnologias

CORDIS: Ciências Tecnológicas > Engenharia > Engenharia química

Outras Informações

ID Authenticus: P-003-MGV

DOI: 10.1016/j.jhazmat.2008.06.014

Abstract (EN): Three-level Box-Behnken factorial design with three factors (pH, temperature and enzyme concentration) combined with response surface methodology (RSM) was applied to optimize the dye degradation of reactive red 239 (RR239), reactive yellow 15 (RY15) and reactive blue 114 (RB114) dyes by commercial laccase. Mathematical models were developed for each dye showing the effect of each factor and their interactions on colour removal. The model predicted for RY15 that a decolourization above 90% (after 24 h) could be obtained when the enzyme concentration, temperature and pH were set at 109.8 U/L, 39.2 degrees C and 6.6, respectively; whilst for RB114 and RR239 the temperature and enzyme concentration did not affect the decolourization (>90%) in the considered range and optimum pH value was found at 5.5-7.0 and 7.0-7.5, respectively. These predicted values were also experimentally validated. Average final values of responses were in good agreement with calculated values, thus confirming the reliability of the models of RY15, RB114 and RR239 decolourization.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6