Determination of E-2-nonenal by high-performance liquid chromatography with UV detection - Assay for the evaluation of beer ageing

• Tipo: Artigo em Revista Científica Internacional
• Data: 2003

Autores

Santos, JR (Autor) FCUP; Carneiro, JR (Autor) Outra; Guido, LF (Autor) FCUP; Almeida, PJ (Autor) FCUP; Rodrigues, JA (Autor) FCUP; Barros, AA (Autor) FCUP

Revista

Título: Journal of Chromatography A

Vol. 985

Páginas: 395-402

ISSN: 0021-9673

Editora: Elsevier

Indexação

ISI Web of Knowledge - 26 Citações

Scopus - 28 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-000-J03

DOI: 10.1016/s0021-9673(02)01396-1

Abstract (EN): The analysis of E-2-nonenal is of considerable interest for the brewery industry as this compound is claimed to be responsible for a paper/cardboard unpleasant flavour. Usually, the presence of E-2-nonenal can be noticed in aged beers at levels higher than 0.1 mug/l. In this work, an analytical method was developed to determine E-2-nonenal in beer involving steam distillation of beer followed by an extraction/concentration step using solid-phase extraction and determination of E-2-nonenal by HPLC with UV detection. Fastness and simplicity are the main advantages of the proposed method, when compared with other existing methodologies for the determination of E-2-nonenal in beer. Using the developed conditions, the interference of E-2-nonenal formed by degradation of its precursors during steam distillation is almost negligible. The presence of sulphur dioxide at legal levels does not interfere with the assay. The method was used in a comparative study of fresh and either naturally or forced aged beers. A much larger chromatographic peak was found near the peak of E-2-nonenal that correlates well with the peak of E-2-nonenal. Identification of the corresponding compound is currently under investigation, considering its future application on the evaluation of beer ageing.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8