Novel Application of Square-Wave Adsorptive-Stripping Voltammetry for the Determination of Xanthohumol in Spent Hops

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Manuela M Moreira (Autor) Outra; Ana M Carvalho (Autor) Outra; Ines M Valente (Autor) Outra; Luis M Goncalves (Autor) Outra; Jose A Rodrigues (Autor) FCUP; Aquiles A Barros (Autor) FCUP; Luis F Guido (Autor) FCUP

Revista

Título: Journal of Agricultural and Food Chemistry

Vol. 59

Páginas: 7654-7658

ISSN: 0021-8561

Editora: American Chemical Society

Indexação

ISI Web of Knowledge - 9 Citações

Scopus - 10 Citações

Classificação Científica

FOS: Ciências agrárias > Outras ciências agrárias

Outras Informações

ID Authenticus: P-002-PKN

DOI: 10.1021/jf2011485

Abstract (EN): This paper reports the development of a novel electrochemical assay for xanthohumol (XN) by square-wave adsorptive-stripping voltammetry (SWAdSV) with a hanging mercury drop electrode. The method showed good repeatability (CV < 2%) and linearity (between 10 and 250 mu g L-1), as well as suitable limits of detection (2.6 mu g L-1) and quantification (8.8 mu g L-1). The method was applied for the quantification of this compound in spent hops, and the results obtained were compared with the HPLC-UV method. XN contents determined by the SWAdSV method were 16 +/- 1 and 100 +/- 4 mu g L-1 for aqueous and methanolic extracts, respectively. The developed new methodology considerably reduces the analysis time, approximately from 25 min (HPLC-UV method) to 7 min, enabling a high sample throughput. In addition, the detection and quantification limits were approximately 5-fold lower than those obtained with the chromatographic method.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5