Hydrogenases in Nostoc sp. strain PCC 73102, a strain lacking a bidirectional enzyme

• Tipo: Artigo em Revista Científica Internacional
• Data: 1997

Autores

Tamagnini, P (Autor) FCUP; Troshina, O (Autor) Outra; Oxelfelt, F (Autor) Outra; Salema, R (Autor) Outra; Lindblad, P (Autor) Outra

Revista

Título: Applied and Environmental Microbiology

Vol. 63

Páginas: 1801-1807

ISSN: 0099-2240

Editora: American Society for Microbiology

Indexação

ISI Web of Knowledge - 93 Citações

Scopus - 97 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-001-BF9

Abstract (EN): The present study was carried out in order to examine and characterize the bidirectional hydrogenase in the cyanobacterium Nostoc sp. strain PCC 73102, Southern hybridizations with the probes Av1 and Av3 (hoxY and hoxH, bidirectional hydrogenase small and large subunits, respectively) revealed the occurrence of corresponding sequences in Anabaena variabilis (control), Anabaena sp. strain PCC 7120, and Nostoc muscorum but not in Nostoc sp. strain PCC 73102, As a control, hybridizations with the probe hup2 (hupL, uptake hydrogenase large subunit) demonstrated the presence of a corresponding gene in all the cyanobacteria tested, including Nostoc sp. strain PCC 73102, Moreover, with three different growth media, a bidirectional enzyme that was functional in vivo was observed in N. muscorum, Anabaena sp. strain PCC 7120, and A. variabilis, whereas Nostoc sp. strain PCC 73102 consistently lacked any detectable in vivo activity, Similar results were obtained when assaying for the presence of an enzyme that is functional in vitro, Native polyacrylamide gel electrophoresis followed by in situ hydrogenase activity staining was used to demonstrate the presence or absence of a functional enzyme, Again, bands corresponding to hydrogenase activity were observed for N. muscorum, Anabaena sp. strain PCC 7120, and A. variabilis but not for Nostoc sp. strain PCC 73102, In conclusion, me were unable to detect a bidirectional hydrogenase in Nostoc sp. strain PCC 73102 with specific physiological and molecular techniques, The same techniques clearly showed the presence of an inducible bidirectional enzyme and corresponding structural genes in N. muscorum, Anabaena sp. strain PCC 7120, and A. variabilis. Hence, Nostoc sp. strain PCC 73102 seems to be an unusual cyanobacterium and an interesting candidate for future biotechnological applications.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7