Total Cholesterol and Desmosterol Contents in Raw, UHT, Infant Formula Powder and Human Milks Determined by a New Fast Micro-HPLC Method

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Heryka Myrna M Ramalho (Autor) Outra; Susana Casal (Autor) FFUP; Beatriz B P P Prior Pinto Oliveira (Autor) FFUP

Revista

Título: Food Analytical Methods

Vol. 4

Páginas: 424-430

ISSN: 1936-9751

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 20 Citações

Scopus - 24 Citações

Classificação Científica

FOS: Ciências agrárias > Outras ciências agrárias

Outras Informações

ID Authenticus: P-002-NHM

DOI: 10.1007/s12161-010-9182-0

Abstract (EN): A simple, fast, sensitive, and environmental friendly micro-method is described for the determination of desmosterol and cholesterol in milk samples using isocratic normal phase high performance liquid chromatography and diode array detection. After sample micro-saponification, the chromatographic separation is achieved in 10 min, further reduced to 6 min when only cholesterol is expected to be present. The method is accurate, presenting cholesterol and desmosterol recoveries > 96% and inter-day RSD lower than 3%. Quantification limits were clearly below the requirements for any milk sample, with 40 and 20 mu g/100 mL for cholesterol and desmosterol, respectively. Several raw and UHT commercial milk samples, infant powder formulas, and human breast milks were analyzed, with cholesterol and desmosterol (present only in the latter) contents within reported amounts. The proposed method proved to be simple and feasible for determination of a large number of samples, requiring less solvent consumption (9-13 mL of hexane for all saponification/extraction/chromatographic steps) and simpler equipment than most ones reported, and a further possibility of partial solvent recycling due to the isocratic chromatographic mode.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7