Characterization and Modulation of Glucose Uptake in a Human Blood-Brain Barrier Model

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Manuela Meireles (Autor) FMUP; Fatima Martel (Autor) FMUP; Joao Araujo (Autor) Outra; Celestino Santos Buelga (Autor) Outra; Susana Gonzalez Manzano (Autor) Outra; Montserrat Duenas (Autor) Outra; Victor de Freitas (Autor) FCUP; Nuno Mateus (Autor) FCUP; Conceicao Calhau (Autor) FMUP; Ana Faria (Autor) FCUP

Revista

Título: Journal of Membrane Biology

Vol. 246

Páginas: 669-677

ISSN: 0022-2631

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 19 Citações

Scopus - 22 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

Outras Informações

ID Authenticus: P-006-7JZ

DOI: 10.1007/s00232-013-9583-2

Abstract (EN): The blood-brain barrier (BBB) plays a key role in limiting and regulating glucose access to glial and neuronal cells. In this work glucose uptake on a human BBB cell model (the hCMEC/D3 cell line) was characterized. The influence of some hormones and diet components on glucose uptake was also studied. H-3-2-deoxy-d-glucose ([H-3]-DG) uptake for hCMEC/D3 cells was evaluated in the presence or absence of tested compounds. [H-3]-DG uptake was sodium- and energy-independent. [H-3]-DG uptake was regulated by Ca2+ and calmodulin but not by MAPK kinase pathways. PKC, PKA and protein tyrosine kinase also seem to be involved in glucose uptake modulation. Progesterone and estrone were found to decrease H-3-DG uptake. Catechin and epicatechin did not have any effect, but their methylated metabolites increased [H-3]-DG uptake. Quercetin and myricetin decreased [H-3]-DG uptake, and glucuronic acid-conjugated quercetin did not have any effect. These cells expressed GLUT1, GLUT3 and SGLT1 mRNA.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9