Phylogenetic relationship of potato CAT1 and CAT2 genes, their differential expression in non-photosynthetic organs and during leaf development, and their association with different cellular processes

• Tipo: Artigo em Revista Científica Internacional
• Data: 2006

Autores

Isabel Santos (Autor) Outra; Helena Pires (Autor) Outra; Jose M Almeida (Autor) Outra; Fernanda Fidalgo (Autor) FCUP; Ana Confraria (Autor) Outra; Marcia Duarte (Autor) Outra; Julio Borlido (Autor) Outra; Roberto Salema (Autor) Outra

Revista

Título: Functional Plant Biology

Vol. 33

Páginas: 639-651

ISSN: 1445-4408

Editora: CSIRO Publishing

Indexação

ISI Web of Knowledge - 5 Citações

Scopus - 4 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-004-PQG

DOI: 10.1071/fp06024

Abstract (EN): Plants contain multiple forms of catalase (CAT) and their specific functions remain uncertain. We cloned two potato cDNAs corresponding to CAT1 and CAT2 genes, analysed their phylogenetic relationship, and studied their expression and activity in different organs to gain clues to their functions. Phylogenetic trees and the alignment of CAT cDNA sequences provided evidence that CAT1 and CAT2 genes have high identity to catalases of other solanaceous species, but are not phylogenetically closely related to one another, which contradicts the phylogenetic closeness ascribed to these genes. Northern blot analyses revealed that expression of CAT genes is controlled by leaf developmental phase. CAT2 expression was higher in both very young and senescent leaves, whereas CAT1 mRNA accumulated mainly in mature leaf, where the lowest CAT2 expression occurred. CAT1 and CAT2 are also differentially expressed in root, sprout and petal. Expression and activity patterns are consistent with different physiological roles for CAT1 and CAT2 isoforms. CAT1 is considered to be associated with photorespiration whereas CAT2 would fulfill physiological roles unrelated to this process. CAT2 appears to be a multifunctional isoform, associated with glyoxysomal activity in leaf senescence, other processes in non-photosynthetic organs and defence, functions that in other solanaceous species are fulfilled by two different isoforms.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13