High-performance liquid chromatographic determination of trace amounts of transition metals in parenteral solutions

• Tipo: Artigo em Revista Científica Internacional
• Data: 1998

Autores

Almeida, AA (Autor) FFUP; Jun, X (Autor) Outra; Lima, JLFC (Autor) FFUP

Revista

Título: The Analyst

Vol. 123

Páginas: 1283-1287

ISSN: 0003-2654

Editora: Royal Society of Chemistry

Indexação

ISI Web of Knowledge - 7 Citações

Scopus - 8 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-001-7QT

DOI: 10.1039/a800553b

Abstract (EN): A high-performance liquid chromatographic (HPLC) method for the determination of trace levels of transition metals (Cu, Ph, Zn, Ni, Co, Cd and Mn) in parenteral solutions (large volume parenterals) was developed, The method involves a preconcentration step on a cation-chelating column (a Nova-Pak Cls guard column coated with Chrome Azurol S) and the separation of the different metals on a Nova-Pak C-18 column permanently coated with sodium hexadecanesulfonate, The eluent was a 100 mmol l(-1) tartrate solution of pH 3.1. Colorimetric detection (at 520 nm) after post-column reaction of the metals with 4-(2-pyridylazo)resorcinol was used. The conditions for the preconcentration and separation of the metals (composition, concentration and pH of the eluent) were studied, The repeatability of the results (n = 3) for a concentration level of about 50 mu g l(-1) was between 3.5 % (Cd) and 5.5% (Ni), The limits of quantification for a preconcentrated sample volume of 4 ml, expressed as mu g l(-1), were 0.40 (Cu), 2.1 (Pb), 0.50 (Zn), 0.33 (Ni), 0.23 (Co), 2.5 (Cd) and 2.6 (Mn), The results obtained with the developed HPLC method for ten commercial samples and seven spiked pooled samples were evaluated by comparison with those provided by electrothermal atomic absorption spectrometry (ETAAS), The developed procedure might be a rapid and inexpensive alternative to ETAAS for quality control in the pharmaceutical industry.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5