Immunostimulatory properties of coffee mannans

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Joana Simoes (Autor) Outra; Pedro Madureira (Autor) Outra; Fernando M Nunes (Autor) Outra; Mario do Rosario Domingues (Autor) Outra; Manuel Vilanova (Autor) ICBAS; Manuel A Coimbra (Autor) Outra

Revista

Título: Molecular Nutrition and Food Research

Vol. 53

Páginas: 1036-1043

ISSN: 1613-4125

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 51 Citações

Scopus - 58 Citações

Classificação Científica

FOS: Ciências agrárias > Outras ciências agrárias

Outras Informações

ID Authenticus: P-003-H8J

DOI: 10.1002/mnfr.200800385

Abstract (EN): Coffee infusion mannans are acetylated polysaccharides containing single Galp and Araf residues as side chains of a beta-(1 -> 4)-Manp backbone. These mannans are structurally similar to the bioactive acetylated mannans from Aloe vera (AV). In this study, acetylated mannans were obtained from two coffee infusions prepared from light and dark roasted beans. These samples were tested for their immunostimulatory activity and compared with an extract of AV mannan and with locust bean gum (LBG) galactomannans. The coffee samples, as well as the AV extract, stimulated murine B- and T-lymphocytes, as evaluated by the in vitro expression of the surface lymphocyte activation market CD69, more marked on B- than on T-lymphocytes. In coffee samples, contrarily to the AV no proliferative effect was noticed. LBG sample did not show any immunostimulatory activity. Because the material that remains in the residue of the hot water extraction was still very rich in mannans, a sequential extraction was performed and a main fraction was recovered with a 4 M NaOH solution. Because this material was insoluble in water, a partial acetylation was performed. These polysaccharides also showed immunostimulatory activity, opening the possibility of exploitation of coffee infusion and coffee residue as sources of bioactive polysaccharides.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8