Experimental Characterization of a Microfluidic Device Based on Passive Crossflow Filters for Blood Fractionation

• Tipo: Artigo em Revista Científica Internacional
• Data: 2022

Autores

Goncalves, IM (Autor) Outra; Castro, I (Autor) Outra; Barbosa, F (Autor) Outra; Faustino, V (Autor) Outra; Catarino, SO (Autor) Outra; Moita, A (Autor) Outra; Miranda, JM (Autor) FEUP; Minas, G (Autor) Outra; Sousa, PC (Autor) Outra; Lima, R (Autor) Outra

Revista

A Revista está pendente de validação pelos Serviços Administrativos.

Título: PROCESSES

Vol. 26

Página Final: 2698

ISSN: 2227-9717

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 3 Citações

Outras Informações

ID Authenticus: P-00X-QMF

DOI: 10.3390/pr10122698

Abstract (EN): The separation of red blood cells (RBCs) from blood plasma and the analysis of individual RBCs are of great importance, as they provide valuable information regarding the health of their donor. Recent developments in microfluidics and microfabrication have contributed to the fabrication of microsystems with complex features to promote the separation and analysis of RBCs. In this work, the separation capacity of a multi-step crossflow microfluidic device was evaluated by using a blood analogue fluid made by Brij L4 micelles and human RBCs separated from whole blood, suspended in a solution with hematocrits (Ht) of 0.5 and 1%. All the samples collected at the outlets of the device were experimentally analyzed and compared. The absorbance spectrum was also measured for the prepared blood samples. The results indicate that the tested blood analogue fluid has exhibited a flow behavior similar to that of blood. In addition, the optical absorbance spectrophotometry revealed that it was possible to evaluate the separation efficiency of the microfluidic device, concluding that the concentration of cells was lower at the most lateral outside outlets of the microchannel due to the cumulative effect of the multiple cross-flow filters.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12