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Automatic flow procedure for the determination of glycerol in wine using enzymatic reaction and spectrophotometry

Título
Automatic flow procedure for the determination of glycerol in wine using enzymatic reaction and spectrophotometry
Tipo
Artigo em Revista Científica Internacional
Ano
2004
Autores
Elizabeth Nunes Fernandes
(Autor)
Outra
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Mariele Nair de Campos Moura
(Autor)
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Boaventura F. Reis
(Autor)
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Revista
Título: Mikrochimica ActaImportada do Authenticus Pesquisar Publicações da Revista
Vol. 77 2
Páginas: 107-112
ISSN: 0026-3672
Editora: Springer Nature
Indexação
Classificação Científica
FOS: Ciências médicas e da saúde > Outras ciências médicas
CORDIS: Ciências da Saúde
Outras Informações
ID Authenticus: P-000-999
Resumo (PT): An automatic flow procedure for the determination of glycerol in wines by employing a flow system based on multicommutation and enzymatic reaction is described. Glycerol dehydrogenase was immobilized on aminopropyl glass beads and packed into a column that was coupled to the flow system. The NADH produced by the enzymatic reaction was monitored by spectrophotometry at 340 nm and its radiation absorption presented a relationship with glycerol concentration. The system manifold comprised a set of three-way solenoid valves controlled by a microcomputer, which was furnished with electronic interfaces and runs a software that was designed to carry out on-line sample dilution, reagent addition, and data acquisition. The procedure allows the determination of glycerol in wine samples without any prior pretreatment. The procedure presented as profitable features a linear response range between 2.0 and 10.0 g l−1 glycerol (R=0.998), a detection limit of 0.006 g l−1 glycerol, a relative standard deviation of 1.8% (n=14) for a typical wine sample presenting 5.3 g l−1 glycerol, a sampling throughput of 33 determinations per hour, and a NAD+ consumption of 0.8 mg per determination. The results were compared with those obtained using a reference method and no significant difference at 90% confidence level was observed. <br> <br> Keywords: Glycerol; Wine; On-line dilution; Multicommutation; Flow analysis; Glycerol dehydrogenase; Spectrophotometry <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6W6H-4CBV1BN-1&_user=2460038&_coverDate=08%2F31%2F2004&_rdoc=5&_fmt=high&_orig=browse&_srch=doc-info(%23toc%236599%232004%23999229997%23507262%23FLA%23display%23Volume)&_cdi=6599&_sort=d&_docanchor=&_ct=19&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=99c97ae057bdb82bf70594adb9412291"> Texto integral</a> <br> <br>
Abstract (EN): An automatic flow procedure for the determination of glycerol in wines by employing a flow system based on multicommutation and enzymatic reaction is described. Glycerol dehydrogenase was immobilized on aminopropyl glass beads and packed into a column that was coupled to the flow system. The NADH produced by the enzymatic reaction was monitored by spectrophotometry at 340 nm and its radiation absorption presented a relationship with glycerol concentration. The system manifold comprised a set of three-way solenoid valves controlled by a microcomputer, which was furnished with electronic interfaces and runs a software that was designed to carry out on-line sample dilution, reagent addition, and data acquisition. The procedure allows the determination of glycerol in wine samples without any prior pretreatment. The procedure presented as profitable features a linear response range between 2.0 and 10.0 g l−1 glycerol (R=0.998), a detection limit of 0.006 g l−1 glycerol, a relative standard deviation of 1.8% (n=14) for a typical wine sample presenting 5.3 g l−1 glycerol, a sampling throughput of 33 determinations per hour, and a NAD+ consumption of 0.8 mg per determination. The results were compared with those obtained using a reference method and no significant difference at 90% confidence level was observed. <br> <br> Keywords: Glycerol; Wine; On-line dilution; Multicommutation; Flow analysis; Glycerol dehydrogenase; Spectrophotometry <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6W6H-4CBV1BN-1&_user=2460038&_coverDate=08%2F31%2F2004&_rdoc=5&_fmt=high&_orig=browse&_srch=doc-info(%23toc%236599%232004%23999229997%23507262%23FLA%23display%23Volume)&_cdi=6599&_sort=d&_docanchor=&_ct=19&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=99c97ae057bdb82bf70594adb9412291"> Full text</a> <br> <br>
Idioma: Português
Tipo (Avaliação Docente): Científica
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