Simple and reproducible HPLC-DAD-ESI-MS/MS analysis of alkaloids in Catharanthus roseus roots

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Federico Ferreres (Autor) Outra; David M Pereira (Autor) Outra; Patricia Valentao (Autor) FFUP; Jorge M A Oliveira (Autor) FFUP; Joana Faria (Autor) Outra; Luis Gaspar (Autor) Outra; Mariana Sottomayor (Autor) FCUP; Paula B Andrade (Autor) FFUP

Revista

Título: Journal of Pharmaceutical and Biomedical Analysis

Vol. 51 Nº 1

Páginas: 65-69

ISSN: 0731-7085

Editora: Elsevier

Indexação

ISI Web of Knowledge - 38 Citações

Scopus - 45 Citações

Pubmed / Medline

Classificação Científica

FOS: Ciências exactas e naturais > Química

CORDIS: Ciências da Saúde

Outras Informações

ID Authenticus: P-003-9SE

DOI: 10.1016/j.jpba.2009.08.005

Resumo (PT): Catharanthus roseus is one of the most important medicinal plants worldwide. The leaves of this species are the only source of the indolomonoterpenic alkaloids vincristin (leurocristine) and vinblastin (vincaleucoblastine), whose anticancer activity represents powerful therapeutics to many diseases, such as Hodgkin lymphoma. Usually, the remaining plant parts go to waste. Here we describe a phytochemical study on this species roots. Alkaloids in aqueous extracts, the usual form of consumption of this matrix, were studied using HPLC–DAD–ESI-MS/MS, which allowed the identification of 19-S-vindolinine, vindolinine, ajmalicine and an ajmalicine isomer, tabersonine, catharanthine, serpentine and a serpentine isomer. Quantification of the identified compounds revealed that serpentine and its isomer were predominant (64.7%) over the other alkaloids, namely vindolinine and its isomer (23.9%), catharanthine (7.7%) and ajmalicine (3.8%). The used procedure revealed to be simple, sensitive and reproducible. <br> <br> Keywords: Catharanthus roseus; Roots; HPLC–DAD–ESI-MS/MS; Alkaloids <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TGX-4X087D9-4&_user=2460038&_coverDate=01%2F05%2F2010&_rdoc=11&_fmt=high&_orig=browse&_srch=doc-info(%23toc%235266%232010%23999489998%231520102%23FLA%23display%23Volume)&_cdi=5266&_sort=d&_docanchor=&_ct=46&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=724b69965c2e77abccd341170b343a6b"> Texto integral </a> <br> <br>

Abstract (EN): Catharanthus roseus is one of the most important medicinal plants worldwide. The leaves of this species are the only source of the indolomonoterpenic alkaloids vincristin (leurocristine) and vinblastin (vincaleucoblastine), whose anticancer activity represents powerful therapeutics to many diseases, such as Hodgkin lymphoma. Usually, the remaining plant parts go to waste. Here we describe a phytochemical study on this species roots. Alkaloids in aqueous extracts, the usual form of consumption of this matrix, were studied using HPLC-DAD-ESI-MS/MS, which allowed the identification of 19-S-vindolinine, vindolinine, ajmalicine and an ajmalicine isomer, tabersonine, catharanthine, serpentine and a serpentine isomer. Quantification of the identified compounds revealed that serpentine and its isomer were predominant (64.7%) over the other alkaloids, namely vindolinine and its isomer (23.9%), catharanthine (7.7%) and ajmalicine (3.8%). The used procedure revealed to be simple, sensitive and reproducible.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5