eRNAs Are Required for p53-Dependent Enhancer Activity and Gene Transcription

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Melo, CA (Autor) Outra; Drost, J (Autor) Outra; Wijchers, PJ (Autor) Outra; van de Werken, H (Autor) Outra; de Wit, E (Autor) Outra; Vrielink, JAFO (Autor) Outra; Elkon, R (Autor) Outra; Melo, SA (Autor) Outra; Leveille, N (Autor) Outra; Kalluri, R (Autor) Outra; de Laat, W (Autor) Outra; Agami, R (Autor) Outra

Revista

A Revista está pendente de validação pelos Serviços Administrativos.

Título: MOLECULAR CELL

Vol. 49

Páginas: 524-535

ISSN: 1097-2765

Indexação

ISI Web of Knowledge - 377 Citações

Scopus - 385 Citações

Outras Informações

ID Authenticus: P-002-0J2

DOI: 10.1016/j.molcel.2012.11.021

Abstract (EN): Binding within or nearby target genes involved in cell proliferation and survival enables the p53 tumor suppressor gene to regulate their transcription and cell-cycle progression. Using genome-wide chromatin-binding profiles, we describe binding of p53 also to regions located distantly from any known p53 target gene. Interestingly, many of these regions possess conserved p53-binding sites and all known hallmarks of enhancer regions. We demonstrate that these p53-bound enhancer regions (p53BERs) indeed contain enhancer activity and interact intrachromosomally with multiple neighboring genes to convey long-distance p53-dependent transcription regulation. Furthermore, p53BERs produce, in a p53-dependent manner, enhancer RNAs (eRNAs) that are required for efficient transcriptional enhancement of interacting target genes and induction of a p53-dependent cell-cycle arrest. Thus, our results ascribe transcription enhancement activity to p53 with the capacity to regulate multiple genes from a single genomic binding site. Moreover, eRNA production from p53BERs is required for efficient p53 transcription enhancement.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12