3D Printed Magneto-Active Microfiber Scaffolds for Remote Stimulation and Guided Organization of 3D In Vitro Skeletal Muscle Models

• Tipo: Artigo em Revista Científica Internacional
• Data: 2023

Autores

Cedillo Servin, G (Autor) Outra; Dahri, O (Autor) Outra; Meneses, J (Autor) Outra; van Duijn, J (Autor) Outra; Moon, H (Autor) Outra; Sage, F (Autor) Outra; Silva, J (Autor) Outra; pereira, a. m. (Autor) FCUP; Magalhães, F. D. (Autor) FEUP; Malda, J (Autor) Outra; Geijsen, N (Autor) Outra; Pinto, AM (Autor) Outra; Castilho, M (Autor) Outra

Revista

Título: Small

Vol. 20

ISSN: 1613-6810

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00Z-CN3

DOI: 10.1002/smll.202307178

Abstract (EN): This work reports the rational design and fabrication of magneto-active microfiber meshes with controlled hexagonal microstructures via melt electrowriting (MEW) of a magnetized polycaprolactone-based composite. In situ iron oxide nanoparticle deposition on oxidized graphene yields homogeneously dispersed magnetic particles with sizes above 0.5 mu m and low aspect ratio, preventing cellular internalization and toxicity. With these fillers, homogeneous magnetic composites with high magnetic content (up to 20 weight %) are obtained and processed in a solvent-free manner for the first time. MEW of magnetic composites enabled the creation of skeletal muscle-inspired design of hexagonal scaffolds with tunable fiber diameter, reconfigurable modularity, and zonal distribution of magneto-active and nonactive material, with elastic tensile deformability. External magnetic fields below 300 mT are sufficient to trigger out-of-plane reversible deformation. In vitro culture of C2C12 myoblasts on three-dimensional (3D) Matrigel/collagen/MEW scaffolds showed that microfibers guided the formation of 3D myotube architectures, and the presence of magnetic particles does not significantly affect viability or differentiation rates after 8 days. Centimeter-sized skeletal muscle constructs allowed for reversible, continued, and dynamic magneto-mechanical stimulation. Overall, these innovative microfiber scaffolds provide magnetically deformable platforms suitable for dynamic culture of skeletal muscle, offering potential for in vitro disease modeling. Formation of skeletal muscle tissue requires mechanical stimulation and proper organization of cell components. Here, microfiber scaffolds are fabricated by melt electrowriting of a magnetized polymer and embedded in cell-loaded hydrogels to create skeletal muscle-like tissues. These flexible fibers guide cell organization and allow for the cyclical mechanical activation of the muscle tissues under external magnetic fields.image

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 16