Increased Galactosidase Beta 1 Expression as a Senescent Key Factor in beta-Cells Function Modulation at the Early Steps of Type 2 Diabetes

• Tipo: Artigo em Revista Científica Internacional
• Data: 2023

Autores

Maduro, AT (Autor) Outra; Pinto, A (Autor) Outra; Ferreira-Gomes J. (Autor) FMUP; Costa, R (Autor) Outra; soares, r (Autor) FMUP; Luis, C (Autor) Outra

Revista

Título: EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES

Vol. 131

Páginas: 282-289

ISSN: 0947-7349

Indexação

ISI Web of Knowledge - 0 Citações

Scopus

Outras Informações

ID Authenticus: P-00Y-D7N

DOI: 10.1055/a-2044-8873

Abstract (EN): Background In type 2 diabetes, insulin resistance is observed, and beta-cells are incapable of responding to glycemia demands, leading to hyperglycemia. Although the nature of beta-cells dysfunction in this disease is not fully understood, a link between the induction of pancreatic beta-cell premature senescence and its metabolic implications has been proposed. This study aimed to understand the relationship between diabetes and pancreatic senescence, particularly at the beginning of the disease. Methods C57Bl/6 J mice were fed two different diets, a normal diet and a high-fat diet, for 16 weeks. Pancreatic histomorphology analysis, insulin quantification, inflammation parameters, and senescence biomarkers for the experimental animals were assessed at weeks 12 and 16. Results The results proved that diabetes onset occurred at week 16 in the High Fat Diet group, supported by glycaemia, weight and blood lipid levels. Increased beta-cells size and number accompanied by increased insulin expression were observed. Also, an inflammatory status of the diabetic group was noted by increased levels of systemic IL-1 beta and increased pancreatic fibrosis. Finally, the expression of galactosidase-beta 1 (GLB1) was significantly increased in pancreatic beta-cells. Conclusion The study findings indicate that senescence, as revealed by an increase in GLB1 expression, is a key factor in the initial stage of diabetes.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8