A NOS1 gene polymorphism associated with asthma and specific immunoglobulin e response to mite allergens in a Colombian population

• Tipo: Artigo em Revista Científica Internacional
• Data: 2007

Autores

Martinez, B (Autor) Outra; Barrios, K (Autor) Outra; Vergara, C (Autor) Outra; Mercado, D (Autor) Outra; Jimenez, S (Autor) Outra; Gusmao, L (Autor) Outra; Caraballo, L (Autor) Outra

Revista

Título: International Archives of Allergy and Immunology

Vol. 144

Páginas: 105-113

ISSN: 1018-2438

Editora: Karger

Indexação

ISI Web of Knowledge - 22 Citações

Scopus - 25 Citações

Outras Informações

ID Authenticus: P-004-DGR

DOI: 10.1159/000103221

Abstract (EN): Background: Nitric oxide (NO) is involved in asthma pathogenesis and is synthesized by three isoforms of NO synthase, one of them encoded by NOS1 gene. The CA-repeat and the C5266T SNP in NOS1 exon 29 have been associated with asthma and IgE levels. We thought to test the association of asthma and asthma-related phenotypes with the exon 29 CA-repeat and the C5266T SNP in a Colombian population sample. Methods: The CA-repeat and the C5266T SNP were genotyped in 167 asthmatics and 166 controls using PCR-based fragment length polymorphism and TaqMan assay. We also determined total and mite-specific IgE against Blomia tropicalis and Dermatophagoides pteronyssinus Results: Three new CA-repeat alleles ,14, 23 and 24 repeats were detected. Allele comprising 16 repeats was associated with asthma (OR: 1.90 (CI 1.22 - 2.97, p(c) = 0.028) and low total (p(c) = 0.02) and specific IgE to B. tropicalis (p(c) < 0.0001) and D. pteronyssinus (p(c) < 0.0001). We found no association of the C5266T SNP and asthma or IgE levels. Conclusion: NOS1 exon 29 CA-repeat may be a risk factor for asthma susceptibility and mite specific IgE response in a Colombian population. Copyright (c) 2007 S. Karger AG, Basel.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9