A Regulatory Switch Alters Chromosome Motions at the Metaphase-to-Anaphase Transition

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

Su, KC (Autor) Outra; Barry, Z (Autor) Outra; Schweizer, N (Autor) Outra; Helder Maiato (Autor) FMUP; Bathe, M (Autor) Outra; Cheeseman, IM (Autor) Outra

Revista

Título: Cell Reports

Vol. 17

Páginas: 1728-1738

ISSN: 2211-1247

Editora: Elsevier

Indexação

ISI Web of Knowledge - 18 Citações

Scopus - 19 Citações

Outras Informações

ID Authenticus: P-00M-6F7

DOI: 10.1016/j.celrep.2016.10.046

Abstract (EN): To achieve chromosome segregation during mitosis, sister chromatids must undergo a dramatic change in their behavior to switch from balanced oscillations at the metaphase plate to directed poleward motion during anaphase. However, the factors that alter chromosome behavior at the metaphase-to-anaphase transition remain incompletely understood. Here, we perform time-lapse imaging to analyze anaphase chromosome dynamics in human cells. Using multiple directed biochemical, genetic, and physical perturbations, our results demonstrate that differences in the global phosphorylation states between metaphase and anaphase are the major determinant of chromosome motion dynamics. Indeed, causing a mitotic phosphorylation state to persist into anaphase produces dramatic metaphase-like oscillations. These induced oscillations depend on both kinetochore-derived and polar ejection forces that oppose poleward motion. Thus, our analysis of anaphase chromosome motion reveals that dephosphorylation of multiple mitotic substrates is required to suppress metaphase chromosome oscillatory motions and achieve directed poleward motion for successful chromosome segregation.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11