NUCLEOLAR ALTERATIONS INDUCED BY 4-AMINOPYRAZOLO(3,4-D)PYRIMIDINE IN ADRENAL-CORTEX AND LIVER-CELLS OF RAT

• Tipo: Artigo em Revista Científica Internacional
• Data: 1987

Autores

Almeida, Henrique (Autor) FMUP; MAGALHAES, M (Autor) Outra; MAGALHAES, MC (Autor) Outra

Revista

Título: Cell and Tissue Research

Vol. 248

Páginas: 231-234

ISSN: 0302-766X

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 3 Citações

Scopus - 2 Citações

Outras Informações

ID Authenticus: P-001-VCQ

DOI: 10.1007/bf01239986

Abstract (EN): The nucleolar ultrastructural changes produced in adrenal zona fasciculata cells and hepatocytes of rats by 4-aminopyrazolo(3,4-d) pyrimidine (4-APP), an inhibitor of the lipoprotein synthesis, are described. Male rats were injected intraperitoneally for three consecutive days with 4-APP in sodium phosphate buffer (50 mg/Kg/day). On the 4th day, the animals were sacrificed and their adrenals and liver were processed for EM. The nucleoli of the adrenal zona fasciculata cells showed nucleolar fragmentation with loss of their normal reticular appearance, separation of fibrillar and granular components, extensive vacuolization and evidence of fibrillar centers. The hepatocyte nucleoli also exhibited fragmentation. These changes seem to be the morphological counterpart of alterations in rRNA synthesis and processing, since 4-APP inhibits "de novo" purine synthesis and thus interferes with its incorporation into RNA and DNA. © 1987 Springer-Verlag.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 4