Hydrolysis of alpha-lactalbumin by cardosin A immobilized on highly activated supports

• Tipo: Artigo em Revista Científica Internacional
• Data: 2003

Autores

Barros, RM (Autor) Outra; Extremina, CI (Autor) Outra; Goncalves, IC (Autor) Outra; Braga, BO (Autor) Outra; Balcao, VM (Autor) Outra; F. Xavier Malcata (Autor) Outra

Revista

Título: Enzyme and Microbial Technology

Vol. 33

Páginas: 908-916

ISSN: 0141-0229

Editora: Elsevier

Indexação

ISI Web of Knowledge - 15 Citações

Scopus - 27 Citações

Outras Informações

ID Authenticus: P-000-E5M

DOI: 10.1016/s0141-0229(03)00244-8

Abstract (EN): In the present research effort, production of derivatives of cardosin A (a plant protease) encompassing full stabilization of its dimeric structure has been achieved, via covalent, multi-subunit immobilization onto highly activated agarose-glutaraldehyde supports. Boiling such enzyme derivatives in the presence of sodium dodecyl sulfate and beta-mercaptoethanol did not lead to leaching of enzyme, thus providing evidence for the effectiveness of the attachment procedure. Furthermore, the cardosin A derivatives prepared under optimal conditions presented ca. half the specific activity of the enzyme in soluble form, and were successfully employed at laboratory-scale trials to perform (selective) hydrolysis of alpha-lactalbumin (alpha-La), one of the major proteins in bovine whey. Hydrolysates of alpha-La were assayed for by the OPA method, as well as by FPLC, SDS-PAGE and HPLC. Thermal inactivation of the immobilized cardosin A was also assessed at 40, 50 and 55 degreesC; at these temperatures, no thermal denaturation took place during incubation for 48 h. The highest degree of hydrolysis was attained by 5 h reaction, at 55 degreesC and pH 5.2. SDS-PAGE of alpha-La hydrolysates displayed bands corresponding to low molecular weight peptides. Our results suggest that cardosin A in immobilized form is a good candidate to bring about proteolysis in the dairy industry, namely in whey processing.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9