Biodiversity and characterization of Staphylococcus species isolated from a small manufacturing dairy plant in Portugal

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Soares, JC (Autor) Outra; Rosario Marques, MR (Autor) Outra; Tavaria, FK (Autor) Outra; Pereira, JO (Autor) Outra; F. Xavier Malcata (Autor) Outra; Pintado, MM (Autor) Outra

Revista

Título: International Journal of Food Microbiology

Vol. 146

Páginas: 123-129

ISSN: 0168-1605

Editora: Elsevier

Indexação

ISI Web of Knowledge - 26 Citações

Scopus - 33 Citações

Outras Informações

ID Authenticus: P-002-T4V

DOI: 10.1016/j.ijfoodmicro.2011.02.008

Abstract (EN): The level and the diversity of the staphylococcal community occurring in the environment and dairy products of a small manufacturing dairy plant were investigated. Species identification was performed using different molecular methods, viz. Multiplex-PCR, amplified ribosomal DNA restriction analysis (ARDRA), and sodA gene sequencing. The main species encountered corresponded to Staphylococcus equorum (41 isolates, 39.0%), S. saprophyticus (28 isolates, 26.7%) and S. epidermidis (15 isolates, 14.3%). Additionally, low incidence of enterotoxin genes was obtained, with only 9 strains (8.6%) being positive for one or more toxin genes. With regard to antimicrobial resistance, 57.1% of the isolates showed at least resistance against one antibiotic, and 28.6% were multi-resistant, which might accomplish resistance for up to 6 antibiotics simultaneously. These results provided evidence that the presence of Staphylococcus species in dairy environment are mostly represented by S. equorum and S. saprophyticus, and illustrate that carrying antimicrobial resistance genes has become reasonably widespread in cheese and dairy environment.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7