Bioinformatic Tools and Guidelines for the Design of Fluorescence In Situ Hybridization Probes

• Tipo: Capítulo ou Parte de Livro
• Data: 2021

Autores

Helena Teixeira (Autor) Outra; Ana L. Sousa (Autor) Outra; Andreia S. Azevedo (Autor) FEUP

Livro

Título: Fluorescence In-Situ Hybridization (FISH) for Microbial Cells. Methods in Molecular Biology

Páginas: 35-50

ISBN: 978-1-0716-1114-2

ISBN Eletrónico: 978-1-0716-1115-9

Editora: Humana Press

Indexação

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00T-M8A

DOI: 10.1007/978-1-0716-1115-9_3

Abstract (EN): Fluorescence in situ hybridization (FISH) is a well-established technique that allows the detection of microorganisms in diverse types of samples (e.g., clinical, food, environmental samples, and biofilm communities). The FISH probe design is an essential step in this technique. For this, two strategies can be used, the manual form based on multiple sequence alignment to identify conserved regions and programs/software specifically developed for the selection of the sequence of the probe. Additionally, databases/software for the theoretical evaluation of the probes in terms of specificity, sensitivity, and thermodynamic parameters (melting temperature and Gibbs free energy change) are used. The purpose of this chapter is to describe the essential steps and guidelines for the design of FISH probes (e.g., DNA and Nucleic Acid Mimic (NAM) probes), and its theoretical evaluation through the application of diverse bioinformatic tools. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 16