Identification of a Novel Nucleobase-Ascorbate Transporter Family Member in Fish and Amphibians

• Tipo: Artigo em Revista Científica Internacional
• Data: 2019

Autores

Oliveira, D (Autor) Outra; Machado, AM (Autor) Outra; Cardoso, T (Autor) Outra; Lopes Marques, M (Autor) Outra; Filipe F C Castro (Autor) FCUP; Ruivo, R (Autor) Outra

Revista

Título: Fishes

Vol. 4

Página Final: 1

Editora: MDPI

Indexação

ISI Web of Knowledge - 1 Citação

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00R-8A1

DOI: 10.3390/fishes4010001

Abstract (EN): Nucleobase-Ascorbate Transporter (NAT) family includes ascorbic acid, nucleobases, and uric acid transporters: With broad evolutionary distribution. In vertebrates, four members have been previously recognized, the ascorbate transporters Slc23a1 and Slc3a2, the nucleobase transporter Slc23a4 and an orphan transporter Slc23a3. Using phylogenetic and synteny analysis, we identify a fifth member of the vertebrate slc23 complement (slc23a5), present in neopterygians (gars and teleosts) and amphibians, and clarify the evolutionary relationships between the novel gene and known slc23 genes. Further comparative analysis puts forward uric acid as the preferred substrate for Slc23a5. Gene expression quantification, using available transcriptomic data, suggests kidney and testis as major expression sites in Xenopus tropicalis (western clawed frog) and Danio rerio (zebrafish). Additional expression in brain was detected in D. rerio, while in the Neoteleostei Oryzias latipes (medaka) slc23a5 expression is restricted to the brain. The biological relevance of the retention of an extra transporter in fish and amphibians is discussed.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12